LOCUS       sequence-101328-         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Goulas T, Cuppari A, Garcia-Castellanos R, Snipas S, Glockshuber R, 
            Arolas JL, Gomis-Ruth FX
  TITLE     The pCri System: A Vector Collection for Recombinant Protein 
            Expression and Purification.
  JOURNAL   PLoS One. 2014 Nov 11;9(11):e112643. doi: 
            10.1371/journal.pone.0112643. eCollection 2014.
  PUBMED    25386923
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-11-11"; volume: "9(11):e112643. doi"; pages: " 
            10.1371/journal.pone.0112643. eCollection 2014"
COMMENT     Sequence Label: pCri-17a
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     194..213
                     /label=LacI-R
                     /note="LacI, reverse primer"
     protein_bind    616..640
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             674..691
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             701..721
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc
       61 ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca
      121 ctttttcccg cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct
      181 gataagagac accggcatac tctgcgacat cgtataacgt tactggtttc atcaaaatcg
      241 tctccctccg tttgaatatt tgattgatcg taaccagatg aagcactctt tccactatcc
      301 ctacagtgtt atggcttgaa caatcacgaa acaataattg gtacgtacga tctttcagcc
      361 gactcaaaca tcaaatctta caaatgtagt ctttgaaagt attacatatg taagatttaa
      421 atgcaaccgt tttttcggaa ggaaatgatg acctcgtttc caccggaatt agcttggtac
      481 cagctattgt aacataatcg gtacgggggt gaaaaagcta acggaaaagg gagcggaaaa
      541 gaatgatgta agcgtgaaaa attttttatc ttatcacttg aaattggaag ggagattctt
      601 tattataaga attgtggaat tgtgagcgga taacaattcc caattaaagg aggaagtcaa
      661 gatgggcagc agccatcatc atcatcatca cagcagcggc gaaaaccttt acttccaggg
      721 ccccatggcc aaagtgagc
//
