LOCUS       sequence-101329-         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Goulas T, Cuppari A, Garcia-Castellanos R, Snipas S, Glockshuber R, 
            Arolas JL, Gomis-Ruth FX
  TITLE     The pCri System: A Vector Collection for Recombinant Protein 
            Expression and Purification.
  JOURNAL   PLoS One. 2014 Nov 11;9(11):e112643. doi: 
            10.1371/journal.pone.0112643. eCollection 2014.
  PUBMED    25386923
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-11-11"; volume: "9(11):e112643. doi"; pages: " 
            10.1371/journal.pone.0112643. eCollection 2014"
COMMENT     Sequence Label: pCri-18a
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     122..141
                     /label=LacI-R
                     /note="LacI, reverse primer"
     protein_bind    544..568
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg
       61 ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac
      121 cggcatactc tgcgacatcg tataacgtta ctggtttcat caaaatcgtc tccctccgtt
      181 tgaatatttg attgatcgta accagatgaa gcactctttc cactatccct acagtgttat
      241 ggcttgaaca atcacgaaac aataattggt acgtacgatc tttcagccga ctcaaacatc
      301 aaatcttaca aatgtagtct ttgaaagtat tacatatgta agatttaaat gcaaccgttt
      361 tttcggaagg aaatgatgac ctcgtttcca ccggaattag cttggtacca gctattgtaa
      421 cataatcggt acgggggtga aaaagctaac ggaaaaggga gcggaaaaga atgatgtaag
      481 cgtgaaaaat tttttatctt atcacttgaa attggaaggg agattcttta ttataagaat
      541 tgtggaattg tgagcggata acaattccca attaaaggag gaaggatcaa tgattcaaaa
      601 acgaaagcgg acagtttcgt tcagacttgt gcttatgtgc acgctgttat ttgtcagttt
      661 gccgattaca aaaacatcag ccatggccaa agtgagc
//
