LOCUS       sequence-101331-         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Goulas T, Cuppari A, Garcia-Castellanos R, Snipas S, Glockshuber R, 
            Arolas JL, Gomis-Ruth FX
  TITLE     The pCri System: A Vector Collection for Recombinant Protein 
            Expression and Purification.
  JOURNAL   PLoS One. 2014 Nov 11;9(11):e112643. doi: 
            10.1371/journal.pone.0112643. eCollection 2014.
  PUBMED    25386923
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-11-11"; volume: "9(11):e112643. doi"; pages: " 
            10.1371/journal.pone.0112643. eCollection 2014"
COMMENT     Sequence Label: pCri-1a
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     809..832
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             860..880
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gctgtatccg tttacctggg atgccgtacg ttacaacggc aagctgattg cttacccgat
       61 cgctgttgaa gcgttatcgc tgatttataa caaagatctg ctgccgaacc cgccaaaaac
      121 ctgggaagag atcccggcgc tggataaaga actgaaagcg aaaggtaaga gcgcgctgat
      181 gttcaacctg caagaaccgt acttcacctg gccgctgatt gctgctgacg ggggttatgc
      241 gttcaagtat gaaaacggca agtacgacat taaagacgtg ggcgtggata acgctggcgc
      301 gaaagcgggt ctgaccttcc tggttgacct gattaaaaac aaacacatga atgcagacac
      361 cgattactcc atcgcagaag ctgcctttaa taaaggcgaa acagcgatga ccatcaacgg
      421 cccgtgggca tggtccaaca tcgacaccag caaagtgaat tatggtgtaa cggtactgcc
      481 gaccttcaag ggtcaaccat ccaaaccgtt cgttggcgtg ctgagcgcag gtattaacgc
      541 cgccagtccg aacaaagagc tggcaaaaga gttcctcgaa aactatctgc tgactgatga
      601 aggtctggaa gcggttaata aagacaaacc gctgggtgcc gtagcgctga agtcttacga
      661 ggaagagttg gcgaaagatc cacgtattgc cgccactatg gaaaacgccc agaaaggtga
      721 aatcatgccg aacatcccgc agatgtccgc tttctggtat gccgtgcgta ctgcggtgat
      781 caacgccgcc agcggtcgtc agactgtcga tgaagccctg aaagacgcgc agactaattc
      841 gggatctggc agtggttctg agaatcttta ttttcagggc gccatggcca aagtgagc
//
