LOCUS       sequence-101358-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Goulas T, Cuppari A, Garcia-Castellanos R, Snipas S, Glockshuber R, 
            Arolas JL, Gomis-Ruth FX
  TITLE     The pCri System: A Vector Collection for Recombinant Protein 
            Expression and Purification.
  JOURNAL   PLoS One. 2014 Nov 11;9(11):e112643. doi: 
            10.1371/journal.pone.0112643. eCollection 2014.
  PUBMED    25386923
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-11-11"; volume: "9(11):e112643. doi"; pages: " 
            10.1371/journal.pone.0112643. eCollection 2014"
COMMENT     Sequence Label: pCri-8a-Strep
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     221..240
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(260..337)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     543..562
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     646..665
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        646..664
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    665..689
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             704..726
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             745..762
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             772..792
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg cagggccaga ctggaggtgg
       61 caacgccaat cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt
      121 aattcagctc cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa acgtggctgg
      181 cctggttcac cacgcgggaa acggtctgat aagagacacc ggcatactct gcgacatcgt
      241 ataacgttac tggtttcaca ttcaccaccc tgaattgact ctcttccggg cgctatcatg
      301 ccataccgcg aaaggttttg cgccattcga tggtgtccgg gatctcgacg ctctccctta
      361 tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc
      421 gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc
      481 accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca
      541 tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc
      601 acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaattaata cgactcacta
      661 taggggaatt gtgagcggat aacaattccc ctctagaaat aattttgttt aactttaaga
      721 aggagatatc atatgggcag cagccatcat catcatcatc acagcagcgg cgaaaacctt
      781 tacttccagg gcgccatggc caaagtgagc
//
