LOCUS       sequence-101368-        1041 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to 
            purify specific genomic regions of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Fujita T, Fujii H
  TITLE     Efficient isolation of specific genomic regions and identification 
            of associated proteins by engineered DNA-binding molecule-mediated 
            chromatin immunoprecipitation (enChIP) using CRISPR.
  JOURNAL   Biochem Biophys Res Commun. 2013 Sep 13;439(1):132-6. doi: 
            10.1016/j.bbrc.2013.08.013. Epub 2013 Aug 11.
  PUBMED    23942116
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2013.08.013"; journalName: "Biochem Biophys Res 
            Commun"; date: "2013-09-13- 13"; volume: "439"; issue: "1"; pages: 
            "132-6"
COMMENT     Sequence Label: 3xFLAG-dCas9/pCMV-7.1
FEATURES             Location/Qualifiers
     source          1..1041
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(236..256)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggttgtcttc ccaacttgcc ccttgctcca taccaccccc ctccacccca taatattata
       61 gaaggacacc tagtcagaca aaatgatgca acttaatttt attaggacaa ggctggtggg
      121 cactggagtg gcaacttcca gggccaggag aggcactggg gaggggtcac agggatgcca
      181 cccgggatcc tctagagtcg actggtaccg atccggtagg gatcgaaccc tttcacacct
      241 tcctcttctt cttggggtca gccctgctgt ctccaccgag ctgagagagg tcgattcttg
      301 tttcatagag ccccgtaatt gactgatgaa tcagtgtggc gtccaggacc tcctttgtag
      361 aggtgtaccg ctttctgtct atggtggtgt cgaagtactt gaaggctgca ggcgcgccca
      421 agttggtcag agtaaacaag tggataatgt tttctgcctg ctccctgatg ggcttatccc
      481 tgtgcttatt gtaagcagaa agcaccttat cgaggttagc gtcggcgagg atcactcttt
      541 tggagaattc gcttatttgc tcgatgatct catcaaggta gtgtttgtgt tgttccacga
      601 acagctgctt ctgctcatta tcttcgggag accctttgag cttttcatag tggctggcca
      661 gatacaagaa attaacgtat ttagagggca gtgccagctc gttacctttc tgcagctcgc
      721 ccgcactagc gagcattcgt ttccggccgt tttcaagctc aaagagagag tacttgggaa
      781 gcttaatgat gaggtctttt ttgacctctt tatatccttt cgcctcgaga aagtcgatgg
      841 ggtttttttc gaagcttgat cgctccatga ttgtgatgcc cagcagttcc ttgacgcttt
      901 tgagtttttt agacttccct ttctccactt tggccacaac cagtacactg taagcgactg
      961 taggagaatc gaatccgccg tatttcttgg ggtcccaatc ttttttgcgt gcgatcagct
     1021 tgtcgctgtt ccttttcggg a
//
