LOCUS       sequence-101386-        3150 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a SFFV
            promoter and multiple cloning site module. Compatible with MultiSite
            Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR SFFV L1-R5
FEATURES             Location/Qualifiers
     source          1..3150
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    85..208
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(263..282)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(264..282)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(287..303)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(287..303)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(405..424)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             416..1225
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(483..502)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1372..1960
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1861..1880
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2114..2131
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2290..2317)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2409..2495)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2544..2566
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2558..2575
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2559..2575
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2591..2690
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gatccactag taacggccgc cagtgtgctg gaattctgca gatatccatc acactggcgg
       61 ccgctcgagc atgcatctag aggacaactt tgtatacaaa agttgaacga gaaacgtaaa
      121 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact
      181 gtaaaacaca acatatccag tcactatgaa tcaactactt agatggtatt agtgacctgt
      241 actgcagggc ggccgcgata tcccctatag tgagtcgtat tacatggtca tagctgtttc
      301 ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
      361 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
      421 ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata
      481 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta
      541 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
      601 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat
      661 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa
      721 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
      781 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga
      841 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag
      901 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa
      961 acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
     1021 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga
     1081 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca
     1141 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
     1201 tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag
     1261 cattacgctg acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta
     1321 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     1381 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     1441 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     1501 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     1561 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     1621 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     1681 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     1741 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     1801 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     1861 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     1921 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     1981 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     2041 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     2101 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     2161 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     2221 gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt
     2281 agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt
     2341 tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct
     2401 cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag
     2461 gcccagtctt ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc
     2521 gttaacgcta gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa
     2581 gctcgggccc caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat
     2641 tgatgagcaa tgctttttta taatgccaac tttgtacaaa aaagcaggct ggagacccaa
     2701 gcttcgctga aagaccccac ctgtaggttt ggcaagctag ctgcagtaac gccattttgc
     2761 aaggcatgga aaaataccaa accaagaata gagaagttca gatcaagggc gggtacatga
     2821 aaatagctaa cgttgggcca aacaggatat ctgcggtgag cagtttcggc cccggcccgg
     2881 ggccaagaac agatggtcac cgcagtttcg gccccggccc gaggccaaga acagatggtc
     2941 cccagatatg gcccaaccct cagcagtttc ttaagaccca tcagatgttt ccaggctccc
     3001 ccaaggacct gaaatgaccc tgcgccttat ttgaattaac caatcagcct gcttctcgct
     3061 tctgttcgcg cgcttctgct tcccgagctc tataaaagag ctcacaaccc ctcactcggc
     3121 gcgccagtcc tccgattgac tgagtcgccg
//
