LOCUS       sequence-101417-        7069 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing SV40 
            promoter and human codon optimized Cas9 module. Compatible with 
            MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 7069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR SV40-hCas9 L3-L2
FEATURES             Location/Qualifiers
     source          1..7069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(7..106)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(136..155)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(137..155)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(160..176)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(160..176)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(278..297)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             289..1098
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(356..375)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1245..1833
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1734..1753
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1987..2004
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2163..2190)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2282..2368)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2417..2439
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2431..2448
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2432..2448
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2465..2560
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2565..2585)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2570..2899
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2750..2885
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      2912..2921
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2918..7021
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2918..7021
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2918..7021
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7034..7054
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctagataccc agctttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt
       61 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgcag
      121 ggcggccgcg atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
      181 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
      241 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt
      301 caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
      361 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag
      421 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
      481 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
      541 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca
      601 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
      661 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
      721 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
      781 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg
      841 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
      901 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
      961 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     1021 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
     1081 ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg
     1141 ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg
     1201 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     1261 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     1321 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     1381 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     1441 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     1501 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     1561 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     1621 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     1681 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     1741 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     1801 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     1861 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     1921 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     1981 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     2041 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     2101 ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg
     2161 caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg
     2221 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg
     2281 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
     2341 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg
     2401 ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg
     2461 ccccaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag
     2521 caatgctttt ttataatgcc aactttgtat aataaagttg ctgtggaatg tgtgtcagtt
     2581 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     2641 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     2701 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     2761 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     2821 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     2881 aggcctaggc ttttgcaaaa agcttggtac cgccaccatg gacaagaagt actccattgg
     2941 gctcgatatc ggcacaaaca gcgtcggctg ggccgtcatt acggacgagt acaaggtgcc
     3001 gagcaaaaaa ttcaaagttc tgggcaatac cgatcgccac agcataaaga agaacctcat
     3061 tggcgccctc ctgttcgact ccggggagac ggccgaagcc acgcggctca aaagaacagc
     3121 acggcgcaga tatacccgca gaaagaatcg gatctgctac ctgcaggaga tctttagtaa
     3181 tgagatggct aaggtggatg actctttctt ccataggctg gaggagtcct ttttggtgga
     3241 ggaggataaa aagcacgagc gccacccaat ctttggcaat atcgtggacg aggtggcgta
     3301 ccatgaaaag tacccaacca tatatcatct gaggaagaag cttgtagaca gtactgataa
     3361 ggctgacttg cggttgatct atctcgcgct ggcgcatatg atcaaatttc ggggacactt
     3421 cctcatcgag ggggacctga acccagacaa cagcgatgtc gacaaactct ttatccaact
     3481 ggttcagact tacaatcagc ttttcgaaga gaacccgatc aacgcatccg gagttgacgc
     3541 caaagcaatc ctgagcgcta ggctgtccaa atcccggcgg ctcgaaaacc tcatcgcaca
     3601 gctccctggg gagaagaaga acggcctgtt tggtaatctt atcgccctgt cactcgggct
     3661 gacccccaac tttaaatcta acttcgacct ggccgaagat gccaagcttc aactgagcaa
     3721 agacacctac gatgatgatc tcgacaatct gctggcccag atcggcgacc agtacgcaga
     3781 cctttttttg gcggcaaaga acctgtcaga cgccattctg ctgagtgata ttctgcgagt
     3841 gaacacggag atcaccaaag ctccgctgag cgctagtatg atcaagcgct atgatgagca
     3901 ccaccaagac ttgactttgc tgaaggccct tgtcagacag caactgcctg agaagtacaa
     3961 ggaaattttc ttcgatcagt ctaaaaatgg ctacgccgga tacattgacg gcggagcaag
     4021 ccaggaggaa ttttacaaat ttattaagcc catcttggaa aaaatggacg gcaccgagga
     4081 gctgctggta aagcttaaca gagaagatct gttgcgcaaa cagcgcactt tcgacaatgg
     4141 aagcatcccc caccagattc acctgggcga actgcacgct atcctcaggc ggcaagagga
     4201 tttctacccc tttttgaaag ataacaggga aaagattgag aaaatcctca catttcggat
     4261 accctactat gtaggccccc tcgcccgggg aaattccaga ttcgcgtgga tgactcgcaa
     4321 atcagaagag accatcactc cctggaactt cgaggaagtc gtggataagg gggcctctgc
     4381 ccagtccttc atcgaaagga tgactaactt tgataaaaat ctgcctaacg aaaaggtgct
     4441 tcctaaacac tctctgctgt acgagtactt cacagtttat aacgagctca ccaaggtcaa
     4501 atacgtcaca gaagggatga gaaagccagc attcctgtct ggagagcaga agaaagctat
     4561 cgtggacctc ctcttcaaga cgaaccggaa agttaccgtg aaacagctca aagaagacta
     4621 tttcaaaaag attgaatgtt tcgactctgt tgaaatcagc ggagtggagg atcgcttcaa
     4681 cgcatccctg ggaacgtatc acgatctcct gaaaatcatt aaagacaagg acttcctgga
     4741 caatgaggag aacgaggaca ttcttgagga cattgtcctc acccttacgt tgtttgaaga
     4801 tagggagatg attgaagaac gcttgaaaac ttacgctcat ctcttcgacg acaaagtcat
     4861 gaaacagctc aagaggcgcc gatatacagg atgggggcgg ctgtcaagaa aactgatcaa
     4921 tgggatccga gacaagcaga gtggaaagac aatcctggat tttcttaagt ccgatggatt
     4981 tgccaaccgg aacttcatgc agttgatcca tgatgactct ctcaccttta aggaggacat
     5041 ccagaaagca caagtttctg gccaggggga cagtcttcac gagcacatcg ctaatcttgc
     5101 aggtagccca gctatcaaaa agggaatact gcagaccgtt aaggtcgtgg atgaactcgt
     5161 caaagtaatg ggaaggcata agcccgagaa tatcgttatc gagatggccc gagagaacca
     5221 aactacccag aagggacaga agaacagtag ggaaaggatg aagaggattg aagagggtat
     5281 aaaagaactg gggtcccaaa tccttaagga acacccagtt gaaaacaccc agcttcagaa
     5341 tgagaagctc tacctgtact acctgcagaa cggcagggac atgtacgtgg atcaggaact
     5401 ggacatcaat cggctctccg actacgacgt ggatcatatc gtgccccagt cttttctcaa
     5461 agatgattct attgataata aagtgttgac aagatccgat aaaaatagag ggaagagtga
     5521 taacgtcccc tcagaagaag ttgtcaagaa aatgaaaaat tattggcggc agctgctgaa
     5581 cgccaaactg atcacacaac ggaagttcga taatctgact aaggctgaac gaggtggcct
     5641 gtctgagttg gataaagccg gcttcatcaa aaggcagctt gttgagacac gccagatcac
     5701 caagcacgtg gcccaaattc tcgattcacg catgaacacc aagtacgatg aaaatgacaa
     5761 actgattcga gaggtgaaag ttattactct gaagtctaag ctggtctcag atttcagaaa
     5821 ggactttcag ttttataagg tgagagagat caacaattac caccatgcgc atgatgccta
     5881 cctgaatgca gtggtaggca ctgcacttat caaaaaatat cccaagcttg aatctgaatt
     5941 tgtttacgga gactataaag tgtacgatgt taggaaaatg atcgcaaagt ctgagcagga
     6001 aataggcaag gccaccgcta agtacttctt ttacagcaat attatgaatt ttttcaagac
     6061 cgagattaca ctggccaatg gagagattcg gaagcgacca cttatcgaaa caaacggaga
     6121 aacaggagaa atcgtgtggg acaagggtag ggatttcgcg acagtccgga aggtcctgtc
     6181 catgccgcag gtgaacatcg ttaaaaagac cgaagtacag accggaggct tctccaagga
     6241 aagtatcctc ccgaaaagga acagcgacaa gctgatcgca cgcaaaaaag attgggaccc
     6301 caagaaatac ggcggattcg attctcctac agtcgcttac agtgtactgg ttgtggccaa
     6361 agtggagaaa gggaagtcta aaaaactcaa aagcgtcaag gaactgctgg gcatcacaat
     6421 catggagcga tcaagcttcg aaaaaaaccc catcgacttt ctcgaggcga aaggatataa
     6481 agaggtcaaa aaagacctca tcattaagct tcccaagtac tctctctttg agcttgaaaa
     6541 cggccggaaa cgaatgctcg ctagtgcggg cgagctgcag aaaggtaacg agctggcact
     6601 gccctctaaa tacgttaatt tcttgtatct ggccagccac tatgaaaagc tcaaagggtc
     6661 tcccgaagat aatgagcaga agcagctgtt cgtggaacaa cacaaacact accttgatga
     6721 gatcatcgag caaataagcg aattctccaa aagagtgatc ctcgccgacg ctaacctcga
     6781 taaggtgctt tctgcttaca ataagcacag ggataagccc atcagggagc aggcagaaaa
     6841 cattatccac ttgtttactc tgaccaactt gggcgcgcct gcagccttca agtacttcga
     6901 caccaccata gacagaaagc ggtacacctc tacaaaggag gtcctggacg ccacactgat
     6961 tcatcagtca attacggggc tctatgaaac aagaatcgac ctctctcagc tcggtggaga
     7021 cagcagggct gaccccaaga agaagaggaa ggtgtgagcg gccgcgact
//
