LOCUS       sequence-101418-        7073 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing SV40 
            promoter and human codon optimized Cas9 module. Compatible with 
            MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 7073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR SV40-hCas9 L5-L2
FEATURES             Location/Qualifiers
     source          1..7073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(11..110)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(140..159)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(141..159)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(164..180)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(164..180)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(282..301)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             293..1102
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(360..379)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1249..1837
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1738..1757
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1991..2008
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2167..2194)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2286..2372)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2421..2443
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2435..2452
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2436..2452
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2469..2564
                     /gene="mutant version of attL"
                     /label=attL5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2569..2589)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2574..2903
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2754..2889
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      2916..2925
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2922..7025
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2922..7025
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2922..7025
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7038..7058
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctagattact acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
       61 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
      121 gcagggcggc cgcgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg
      181 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
      241 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca
      301 tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg
      361 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg
      421 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
      481 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
      541 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac
      601 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
      661 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
      721 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
      781 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact
      841 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
      901 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
      961 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
     1021 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
     1081 gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat
     1141 tacgctgact tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc
     1201 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1261 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1321 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1381 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1441 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1501 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1561 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     1621 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     1681 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     1741 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     1801 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     1861 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     1921 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     1981 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     2041 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     2101 agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga
     2161 aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat
     2221 ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc
     2281 ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc
     2341 cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt
     2401 aacgctagca tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct
     2461 cgggccccaa ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga
     2521 tgagcaatgc ttttttataa tgccaacttt gtatacaaaa gttgctgtgg aatgtgtgtc
     2581 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     2641 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     2701 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     2761 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     2821 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     2881 ttggaggcct aggcttttgc aaaaagcttg gtaccgccac catggacaag aagtactcca
     2941 ttgggctcga tatcggcaca aacagcgtcg gctgggccgt cattacggac gagtacaagg
     3001 tgccgagcaa aaaattcaaa gttctgggca ataccgatcg ccacagcata aagaagaacc
     3061 tcattggcgc cctcctgttc gactccgggg agacggccga agccacgcgg ctcaaaagaa
     3121 cagcacggcg cagatatacc cgcagaaaga atcggatctg ctacctgcag gagatcttta
     3181 gtaatgagat ggctaaggtg gatgactctt tcttccatag gctggaggag tcctttttgg
     3241 tggaggagga taaaaagcac gagcgccacc caatctttgg caatatcgtg gacgaggtgg
     3301 cgtaccatga aaagtaccca accatatatc atctgaggaa gaagcttgta gacagtactg
     3361 ataaggctga cttgcggttg atctatctcg cgctggcgca tatgatcaaa tttcggggac
     3421 acttcctcat cgagggggac ctgaacccag acaacagcga tgtcgacaaa ctctttatcc
     3481 aactggttca gacttacaat cagcttttcg aagagaaccc gatcaacgca tccggagttg
     3541 acgccaaagc aatcctgagc gctaggctgt ccaaatcccg gcggctcgaa aacctcatcg
     3601 cacagctccc tggggagaag aagaacggcc tgtttggtaa tcttatcgcc ctgtcactcg
     3661 ggctgacccc caactttaaa tctaacttcg acctggccga agatgccaag cttcaactga
     3721 gcaaagacac ctacgatgat gatctcgaca atctgctggc ccagatcggc gaccagtacg
     3781 cagacctttt tttggcggca aagaacctgt cagacgccat tctgctgagt gatattctgc
     3841 gagtgaacac ggagatcacc aaagctccgc tgagcgctag tatgatcaag cgctatgatg
     3901 agcaccacca agacttgact ttgctgaagg cccttgtcag acagcaactg cctgagaagt
     3961 acaaggaaat tttcttcgat cagtctaaaa atggctacgc cggatacatt gacggcggag
     4021 caagccagga ggaattttac aaatttatta agcccatctt ggaaaaaatg gacggcaccg
     4081 aggagctgct ggtaaagctt aacagagaag atctgttgcg caaacagcgc actttcgaca
     4141 atggaagcat cccccaccag attcacctgg gcgaactgca cgctatcctc aggcggcaag
     4201 aggatttcta cccctttttg aaagataaca gggaaaagat tgagaaaatc ctcacatttc
     4261 ggatacccta ctatgtaggc cccctcgccc ggggaaattc cagattcgcg tggatgactc
     4321 gcaaatcaga agagaccatc actccctgga acttcgagga agtcgtggat aagggggcct
     4381 ctgcccagtc cttcatcgaa aggatgacta actttgataa aaatctgcct aacgaaaagg
     4441 tgcttcctaa acactctctg ctgtacgagt acttcacagt ttataacgag ctcaccaagg
     4501 tcaaatacgt cacagaaggg atgagaaagc cagcattcct gtctggagag cagaagaaag
     4561 ctatcgtgga cctcctcttc aagacgaacc ggaaagttac cgtgaaacag ctcaaagaag
     4621 actatttcaa aaagattgaa tgtttcgact ctgttgaaat cagcggagtg gaggatcgct
     4681 tcaacgcatc cctgggaacg tatcacgatc tcctgaaaat cattaaagac aaggacttcc
     4741 tggacaatga ggagaacgag gacattcttg aggacattgt cctcaccctt acgttgtttg
     4801 aagataggga gatgattgaa gaacgcttga aaacttacgc tcatctcttc gacgacaaag
     4861 tcatgaaaca gctcaagagg cgccgatata caggatgggg gcggctgtca agaaaactga
     4921 tcaatgggat ccgagacaag cagagtggaa agacaatcct ggattttctt aagtccgatg
     4981 gatttgccaa ccggaacttc atgcagttga tccatgatga ctctctcacc tttaaggagg
     5041 acatccagaa agcacaagtt tctggccagg gggacagtct tcacgagcac atcgctaatc
     5101 ttgcaggtag cccagctatc aaaaagggaa tactgcagac cgttaaggtc gtggatgaac
     5161 tcgtcaaagt aatgggaagg cataagcccg agaatatcgt tatcgagatg gcccgagaga
     5221 accaaactac ccagaaggga cagaagaaca gtagggaaag gatgaagagg attgaagagg
     5281 gtataaaaga actggggtcc caaatcctta aggaacaccc agttgaaaac acccagcttc
     5341 agaatgagaa gctctacctg tactacctgc agaacggcag ggacatgtac gtggatcagg
     5401 aactggacat caatcggctc tccgactacg acgtggatca tatcgtgccc cagtcttttc
     5461 tcaaagatga ttctattgat aataaagtgt tgacaagatc cgataaaaat agagggaaga
     5521 gtgataacgt cccctcagaa gaagttgtca agaaaatgaa aaattattgg cggcagctgc
     5581 tgaacgccaa actgatcaca caacggaagt tcgataatct gactaaggct gaacgaggtg
     5641 gcctgtctga gttggataaa gccggcttca tcaaaaggca gcttgttgag acacgccaga
     5701 tcaccaagca cgtggcccaa attctcgatt cacgcatgaa caccaagtac gatgaaaatg
     5761 acaaactgat tcgagaggtg aaagttatta ctctgaagtc taagctggtc tcagatttca
     5821 gaaaggactt tcagttttat aaggtgagag agatcaacaa ttaccaccat gcgcatgatg
     5881 cctacctgaa tgcagtggta ggcactgcac ttatcaaaaa atatcccaag cttgaatctg
     5941 aatttgttta cggagactat aaagtgtacg atgttaggaa aatgatcgca aagtctgagc
     6001 aggaaatagg caaggccacc gctaagtact tcttttacag caatattatg aattttttca
     6061 agaccgagat tacactggcc aatggagaga ttcggaagcg accacttatc gaaacaaacg
     6121 gagaaacagg agaaatcgtg tgggacaagg gtagggattt cgcgacagtc cggaaggtcc
     6181 tgtccatgcc gcaggtgaac atcgttaaaa agaccgaagt acagaccgga ggcttctcca
     6241 aggaaagtat cctcccgaaa aggaacagcg acaagctgat cgcacgcaaa aaagattggg
     6301 accccaagaa atacggcgga ttcgattctc ctacagtcgc ttacagtgta ctggttgtgg
     6361 ccaaagtgga gaaagggaag tctaaaaaac tcaaaagcgt caaggaactg ctgggcatca
     6421 caatcatgga gcgatcaagc ttcgaaaaaa accccatcga ctttctcgag gcgaaaggat
     6481 ataaagaggt caaaaaagac ctcatcatta agcttcccaa gtactctctc tttgagcttg
     6541 aaaacggccg gaaacgaatg ctcgctagtg cgggcgagct gcagaaaggt aacgagctgg
     6601 cactgccctc taaatacgtt aatttcttgt atctggccag ccactatgaa aagctcaaag
     6661 ggtctcccga agataatgag cagaagcagc tgttcgtgga acaacacaaa cactaccttg
     6721 atgagatcat cgagcaaata agcgaattct ccaaaagagt gatcctcgcc gacgctaacc
     6781 tcgataaggt gctttctgct tacaataagc acagggataa gcccatcagg gagcaggcag
     6841 aaaacattat ccacttgttt actctgacca acttgggcgc gcctgcagcc ttcaagtact
     6901 tcgacaccac catagacaga aagcggtaca cctctacaaa ggaggtcctg gacgccacac
     6961 tgattcatca gtcaattacg gggctctatg aaacaagaat cgacctctct cagctcggtg
     7021 gagacagcag ggctgacccc aagaagaaga ggaaggtgtg agcggccgcg act
//
