LOCUS       sequence-101435-        4857 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV 
            promoter and tdTomato module. Compatible with MultiSite Gateway 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR CMV tdTomato R4-R3
FEATURES             Location/Qualifiers
     source          1..4857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    9..132
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(188..206)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(211..227)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(211..227)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(329..348)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             340..1149
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(407..426)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1296..1884
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1785..1804
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2038..2055
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2214..2241)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2333..2419)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2468..2490
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2482..2499
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2483..2499
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2549..2673)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     enhancer        2700..3079
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3080..3283
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3234..3254
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        3328..3346
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3379..3388
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3385..4815
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     primer_bind     complement(4836..4852)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4837..4853)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 ctagaggaca actttgtata ataaagttga acgagaaacg taaaatgata taaatatcaa
       61 tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat
      121 ccagtcacta tgaatcaact acttagatgg tattagtgac ctgtactgca gggcggccgc
      181 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
      241 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
      301 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
      361 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
      421 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg
      481 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
      541 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
      601 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta
      661 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
      721 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
      781 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
      841 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
      901 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
      961 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     1021 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     1081 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     1141 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
     1201 cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga
     1261 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1321 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1381 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1441 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1501 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1561 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1621 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     1681 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1741 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     1801 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     1861 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     1921 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     1981 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     2041 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     2101 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     2161 cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc
     2221 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
     2281 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
     2341 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact
     2401 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg
     2461 atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gcccctacag
     2521 gtcactaata ccatctaagt agttgattca tagtgactgg atatgttgtg ttttacagta
     2581 ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta
     2641 cgtttctcgt tcaacttttc tatacaaagt tggcgatgta cgggccagat atacgcgttg
     2701 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     2761 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     2821 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     2881 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     2941 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     3001 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     3061 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     3121 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     3181 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     3241 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga
     3301 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagcttg
     3361 gtaccgagct cggatcccgc caccatggtg agcaagggcg aggaggtcat caaagagttc
     3421 atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc
     3481 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
     3541 ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg
     3601 tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc
     3661 aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc
     3721 tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc
     3781 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac
     3841 ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc
     3901 cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc
     3961 tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg
     4021 gaacagtacg agcgctccga gggccgccac cacctgttcc tggggcatgg caccggcagc
     4081 accggcagcg gcagctccgg caccgcctcc tccgaggaca acaacatggc cgtcatcaaa
     4141 gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga gttcgagatc
     4201 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     4261 aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat gtacggctcc
     4321 aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc cttccccgag
     4381 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac cgtgacccag
     4441 gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg caccaacttc
     4501 ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgc
     4561 ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa gctgaaggac
     4621 ggcggccgct acctggtgga gttcaagacc atctacatgg ccaagaagcc cgtgcaactg
     4681 cccggctact actacgtgga caccaagctg gacatcacct cccacaacga ggactacacc
     4741 atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctgta cggcatggac
     4801 gagctgtaca agtaagaatt cgatatcaag cttatcgata ccgtcgacct cgagcct
//
