LOCUS       sequence-101439-        4507 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a SFFV
            promoter and tdTomato module. Compatible with MultiSite Gateway 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR SFFV tdTomato L5-L2
FEATURES             Location/Qualifiers
     source          1..4507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(9..108)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(138..157)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(139..157)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(162..178)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(162..178)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(280..299)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             291..1100
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(358..377)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1247..1835
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1736..1755
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1989..2006
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2165..2192)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2284..2370)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2419..2441
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2433..2450
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2434..2450
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2467..2562
                     /gene="mutant version of attL"
                     /label=attL5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2618..3019
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      3029..3038
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3035..4465
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     primer_bind     complement(4486..4502)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4487..4503)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 ctagaggtac ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
       61 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctgc
      121 agggcggccg cgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc
      181 agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat
      241 catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata
      301 ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt
      361 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga
      421 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta
      481 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc
      541 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag
      601 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag
      661 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg
      721 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt
      781 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt
      841 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt
      901 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat
      961 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac
     1021 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga
     1081 tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta
     1141 cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt
     1201 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     1261 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     1321 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     1381 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     1441 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     1501 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     1561 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     1621 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     1681 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     1741 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     1801 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     1861 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     1921 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     1981 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     2041 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     2101 cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa
     2161 cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg
     2221 cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg
     2281 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
     2341 gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa
     2401 cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt cttaagctcg
     2461 ggccccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgatg
     2521 agcaatgctt ttttataatg ccaactttgt atacaaaagt tgggagaccc aagcttcgct
     2581 gaaagacccc acctgtaggt ttggcaagct agctgcagta acgccatttt gcaaggcatg
     2641 gaaaaatacc aaaccaagaa tagagaagtt cagatcaagg gcgggtacat gaaaatagct
     2701 aacgttgggc caaacaggat atctgcggtg agcagtttcg gccccggccc ggggccaaga
     2761 acagatggtc accgcagttt cggccccggc ccgaggccaa gaacagatgg tccccagata
     2821 tggcccaacc ctcagcagtt tcttaagacc catcagatgt ttccaggctc ccccaaggac
     2881 ctgaaatgac cctgcgcctt atttgaatta accaatcagc ctgcttctcg cttctgttcg
     2941 cgcgcttctg cttcccgagc tctataaaag agctcacaac ccctcactcg gcgcgccagt
     3001 cctccgattg actgagtcgc cggatcccgc caccatggtg agcaagggcg aggaggtcat
     3061 caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga
     3121 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     3181 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg
     3241 ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc
     3301 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac
     3361 ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa
     3421 cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga
     3481 gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa
     3541 ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca
     3601 actgcccggc tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta
     3661 caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc tggggcatgg
     3721 caccggcagc accggcagcg gcagctccgg caccgcctcc tccgaggaca acaacatggc
     3781 cgtcatcaaa gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga
     3841 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
     3901 gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat
     3961 gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc
     4021 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac
     4081 cgtgacccag gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg
     4141 caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     4201 caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa
     4261 gctgaaggac ggcggccgct acctggtgga gttcaagacc atctacatgg ccaagaagcc
     4321 cgtgcaactg cccggctact actacgtgga caccaagctg gacatcacct cccacaacga
     4381 ggactacacc atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctgta
     4441 cggcatggac gagctgtaca agtaagaatt cgatatcaag cttatcgata ccgtcgacct
     4501 cgagcct
//
