LOCUS       sequence-101469-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Centromeres, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Gao L, Shao L, Higgins CD, Poulton JS, Peifer M, Davidson MW, Wu X, 
            Goldstein B, Betzig E
  TITLE     Noninvasive imaging beyond the diffraction limit of 3D dynamics in 
            thickly fluorescent specimens.
  JOURNAL   Cell. 2012 Dec 7;151(6):1370-85. doi: 10.1016/j.cell.2012.10.008.
  PUBMED    23217717
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.10"; journalName: "Cell"; date: "2012-12-7- 7";
            volume: "151"; issue: "6"; pages: "1370-85"
COMMENT     Sequence Label: tdTomato-CENPB-N-22
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      537..546
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(573..776)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(602..622)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 cgtcgactgc agaattcgaa gcttgagctc gagagtgctc ccgccactgc cctccgaggg
       61 caccgcggcc ggactggcag gcgccgccgg ggtgcggggg gcagcgtttc gcgcgcgggc
      121 gcgggccacg ccgctgcagg acaccacgcc gtggcgccgg cggaagcggt ccagccagcc
      181 gttggaggcg gtgaagtcgt ccatgcccag ctcctcggct atgcgcagcg ccttctcctt
      241 gaggatgatg cccttgaccg gcaggccggc ggcgcggatc tgctggaacc aggcgatgag
      301 caagccctcg agcttgtcgt agggagacag cttgttggtc ttgcggcagg tggaggccac
      361 cccgtacttg cgctccgacg ccaggatggc gcgcttgttc ttcaggatcg tgctcagcgt
      421 ggacggcggg atgttgaagc gccgcgcgat ctcgcccttg cgtaggtccg ggttctcctc
      481 cacctcctgg atgatccgcg acttctcccg gaacgtcagc tgccgccgct tggggcccat
      541 ggtggctagc ggatctgacg gttcactaaa ccagctctgc ttatatagac ctcccaccgt
      601 acacgcctac cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc
      661 cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat
      721 ccccgtgagt caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg
      781 gtaatagcga tgactaatac gtagatgtac tgccaagtag gaa
//
