LOCUS       sequence-101474-        5787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of luciferase driven by truncated mPer2 promoter region 
            (bases -1128 to -141 with respect to transcription start site at 
            +1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Yoo SH, Ko CH, Lowrey PL, Buhr ED, Song EJ, Chang S, Yoo OJ, 
            Yamazaki S, Lee C, Takahashi JS
  TITLE     A noncanonical E-box enhancer drives mouse Period2 circadian 
            oscillations in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Feb 15;102(7):2608-13. Epub 2005 Feb 
            7.
  PUBMED    15699353
REFERENCE   2  (bases 1 to 5787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2005-02-15"; volume: "102(7)"; pages: 
            "2608-13. Epub 2005 Feb 7"
COMMENT     Sequence Label: pGL1
FEATURES             Location/Qualifiers
     source          1..5787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..62)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    1671..1792
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1682..1701)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1736..1755
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2040..2057)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2211..2799)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2291..2310)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2970..3761,3762..3830))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3593..3612
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3831..3935)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3962..4417
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4049..4068)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4259..4280
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    4548..4596
                     /note="synthetic polyadenylation signal"
     primer_bind     4650..4669
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     CDS             5090..5101
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      5759..5768
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5765..5787,1..1630)
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
ORIGIN
        1 gaaaggcccg gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa
       61 ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga
      121 ggtggacatc acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa
      181 acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt
      241 ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat
      301 ttataatgaa cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt
      361 ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa
      421 aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt
      481 cacatctcat ctacctcccg gttttaatga atacgatttt gtgccagagt ccttcgatag
      541 ggacaagaca attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt
      601 cgctctgcct catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg
      661 caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg
      721 aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt
      781 tgaagaagag ctgtttctga ggagccttca ggattacaag attcaaagtg cgctgctggt
      841 gccaacccta ttctccttct tcgccaaaag cactctgatt gacaaatacg atttatctaa
      901 tttacacgaa attgcttctg gtggcgctcc cctctctaag gaagtcgggg aagcggttgc
      961 caagaggttc catctgccag gtatcaggca aggatatggg ctcactgaga ctacatcagc
     1021 tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt
     1081 ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg
     1141 cgaactgtgt gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac
     1201 caacgccttg attgacaagg atggatggct acattctgga gacatagctt actgggacga
     1261 agacgaacac ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca
     1321 ggtggctccc gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg
     1381 tgtcgcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga
     1441 gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac
     1501 cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg
     1561 aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat
     1621 cgccgtgtaa ttctagagtc ggggcggccg gccgcttcga gcagacatga taagatacat
     1681 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     1741 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     1801 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     1861 gtaaaacctc tacaaatgtg gtaaaatcga taaggatccg tcgaccgatg cccttgagag
     1921 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
     1981 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc
     2041 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     2101 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     2161 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2221 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     2281 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2341 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     2401 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     2461 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     2521 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     2581 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     2641 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     2701 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     2761 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     2821 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     2881 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     2941 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     3001 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     3061 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     3121 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     3181 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3241 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     3301 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     3361 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     3421 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     3481 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     3541 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     3601 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     3661 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     3721 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     3781 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     3841 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     3901 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     3961 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4021 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4081 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4141 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     4201 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     4261 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     4321 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     4381 aacgcgaatt ttaacaaaat attaacgttt acaatttccc attcgccatt caggctgcgc
     4441 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagcc caagctacca
     4501 tgataagtaa gtaatattaa ggtacgggag gtacttggag cggccgcaat aaaatatctt
     4561 tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta acatacgctc
     4621 tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg
     4681 caggtgccag aacattctct atcgataggt accgagctcc aagcttgcct tctccatcac
     4741 accttgcatg agtctttagg ttgttctctg tcagcctcaa acccgctccg aggaaacttc
     4801 tactccctcc tttgaccctt tggacaggag cctgaacgct ttagtaggct tccagacagt
     4861 gctcttgaaa gaaccaaata gcttcaacca aggttccaca ggggcagggc tgtcctatca
     4921 ctggaggagt accctcccct gactagctag tgtctgtagc ttccacttca gaaaagccct
     4981 gctgttccag atgcacaccc cgcttccata gttcctgtaa ggttaataaa ctacaccacc
     5041 gcatttggtt aagcttccct gtagaacgtc agtcttctct ccctatgtga ttgagggcag
     5101 gaagaaatca cttctttcct ttgtatctct gcacggcaat tatgacctta tttcctgaat
     5161 caacactaac tagcaacacg cagtttcaga aacaagaaag gctaagtggg agttttgtgc
     5221 tttggcccat ctggaatgac ggtcagcctg gggggcctgt cctagggtca cccagcctgt
     5281 cctgggaagg tgctcagcag cagatccaga ggggccgtcc tatttgtcct caagcgtctc
     5341 gccatgaatg aatgagaggg gaaatgaatg aactgggctg gatgagcgaa aggtgtcagc
     5401 agagagcatt ctcggtcctt cggattaccg aggctggtca cgtcgtcgca ggtgataggc
     5461 cgggggccct ggtctctgcc ggctgtgagt tgcgcagcgg ccaagcacca ttcccccgcg
     5521 ccgcagtggt acgcgccact ccggggctgc acgagcgggc caccgccgtg ccaggtgaat
     5581 ggaagtcccg caggccggaa gtggacgagc ctactcgccc gggcgcgggg gggcgcaaga
     5641 gcgcgcagca tcttcattga ggaacccggg cgggcgaaca tggagttcca tgtgcgtctt
     5701 atgtaaagct cgagatctgc gatctaagta agcttggcat tccggtactg ttggtaaagc
     5761 caccatggaa gacgccaaaa acataaa
//
