LOCUS       sequence-101475-         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Backbone for lentiviral transduction of Massively Parallel Reporter 
            Assay (MPRA) libraries.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  TITLE     Mikkelsen Lab MPRA plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMPRAlenti1
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    16..140
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(165..170,171..188,189..195)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(315..334)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 attctgcaga tatcaacaag tttgtacaaa aaagctgaac gagaaacgta aaatgatata
       61 aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca
      121 caacatatcc agtcactatg gcggccgcat taggcacccc aggctttaca ctttatgctt
      181 ccggctcgta taatgtgtgg attttgagtt aggatccggc gagattttca ggagctaagg
      241 aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc
      301 gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc
      361 agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg
      421 cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga
      481 aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc
      541 aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac
      601 acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt
      661 ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt
      721 taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata
      781 cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtctgtgatg
      841 gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggca
//
