LOCUS       sequence-101490-         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Backbone for lentiviral transduction of Massively Parallel Reporter 
            Assay (MPRA) libraries.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  TITLE     Mikkelsen Lab MPRA plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMPRAlenti1
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    273..397
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(438..743)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(540..559)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 aagaacgttc acggcgacta ctgcacttat atacggttct cccccaccct cgggaaaaag
       61 gcggagccag tacacgacat cactttccca gtttaccccg cgccaccttc tctaggcacc
      121 ggttcaattg ccgacccctc cccccaactt ctcggggact gtgggcgatg tgcgctctgc
      181 ccactgacgg gcaccggagc gatcgcagat ccttttaatt aatgtctgct cgaagcggcc
      241 ggccgccccg actagcttgg ccgccgaggc caaccacttt gtacaagaaa gctgaacgag
      301 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta
      361 cataatactg taaaacacaa catatccagt cactatggtc gacctgcaga ctggctgtgt
      421 ataacggagc ctgacattta tattccccag aacatcaggt taatggcgtt tttgatgtca
      481 ttttcgcggt ggctgagatc agccacttct tccccgataa cggagaccgg cacactggcc
      541 atatcggtgg tcatcatgcg ccagctttca tccccgatat gcaccaccgg gtaaagttca
      601 cgggagactt tatctgacag cagacgtgca ctggccaggg ggatcaccat ccgtcgcccg
      661 ggcgtgtcaa taatatcact ctgtacatcc acaaacagac gataacggct ctctctttta
      721 taggtgtaaa ccttaaactg catttcacca gtccctgttc tcgtcagcaa aagagccgtt
      781 catttcaata aaccgggcga cctcagccat cccttcctga ttttccgctt tccagcgttc
      841 ggcacgcaga cgacgggctt cattctgcat ggttgtgctt accagaccgg agatattgac
      901 atcatatatg ccttgagcaa ctgatagctg tcgctgtcaa ctgtcactgt aatacgctgc
      961 ttcatagcac acctc
//
