LOCUS       sequence-101494-         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human transferrin receptor tagged with pH sensitive red fluorescent 
            protein pHuji.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Shen Y, Rosendale M, Campbell RE, Perrais D
  TITLE     pHuji, a pH-sensitive red fluorescent protein for imaging of exo- 
            and endocytosis.
  JOURNAL   J Cell Biol. 2014 Nov 10;207(3):419-32. doi: 10.1083/jcb.201404107.
  PUBMED    25385186
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2014-11-10- 10"; volume: "207"; 
            issue: "3"; pages: "419-32"
COMMENT     Sequence Label: TfR-pHuji
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..26
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        236..254
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     236..253
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     267..286
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
       61 atagaagaca ccgggaccga tccagcctcc gcggccggga acggtgcatt ggaacgcgga
      121 ttccccgtgc caagagtgac gtaagtaccg cctatagagt ctataggccc acccccttgg
      181 cttcgttaga acgcggctac aattaataca taaccttatg tatcatacac atacgattta
      241 ggtgacacta tagaataaca tccactttgc ctttctctcc acaggtgtcc actcccaggt
      301 ccaactgcac ctcggttcta tcgattgaat tccccgggga tccaccatgg atcaagctag
      361 atcagcattc tctaacttgt ttggtggaga accattgtca tatacccggt tcagcctggc
      421 tcggcaagta gatggcgata acagtcatgt ggagatgaaa cttgctgtag atgaagaaga
      481 aaatgctgac aataacacaa aggccaatgt cacaaaacca aaaaggtgta gtggaagtat
      541 ctgctatggg actattgctg tgatcgtctt tttcttgatt ggatttatga ttggctactt
      601 gggctattgt aaaggggtag aaccaaaaac tgagtgtgag agactggcag gaaccgagtc
      661 tccagtgagg gaggagccag gagaggactt ccctgcagca cgtcgcttat attgggatga
      721 cctgaagaga aagttgtcgg agaaactgga cagcacagac ttcaccagca ccatcaagct
      781 gctgaatgaa aattcatatg tccctcgtga ggctggatct caaaaagatg aaaatcttgc
      841 gttgtatgtt gaaaatcaat ttcgtgaatt taaactcagc aaagtctggc gtgatcaaca
      901 ttttgttaag attcaggtca aagacagcgc tcaaaactcg gtgatcat
//
