LOCUS       sequence-101496-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human transferrin receptor tagged with pH sensitive red fluorescent 
            protein pHuji.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Shen Y, Rosendale M, Campbell RE, Perrais D
  TITLE     pHuji, a pH-sensitive red fluorescent protein for imaging of exo- 
            and endocytosis.
  JOURNAL   J Cell Biol. 2014 Nov 10;207(3):419-32. doi: 10.1083/jcb.201404107.
  PUBMED    25385186
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2014-11-10- 10"; volume: "207"; 
            issue: "3"; pages: "419-32"
COMMENT     Sequence Label: TfR-pHuji
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(52..759)
                     /codon_start=1
                     /product="pH-sensitive red fluorescent protein derived from
                     mApple, for imaging of exocytosis and endocytosis (Shen et 
                     al., 2014)"
                     /label=pHuji
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKYRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(327..346)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 agttgggcca tggcggccaa gcttctgcag gtcgactcta gattagctag ccttgtacag
       61 ctcgtccatg ccgccggtgg agtggcggcc ctcggcgcgt tcgtactgtt ccacgatggt
      121 gtagtcctcg ttgtgggaca cgatgtccaa cttgatgtcc acgatgtagg cgccgggcag
      181 ctgcacgggc ttcttggcct tgtaggtggt cttgacctcg gcggcgtagt ggccgccgtc
      241 cttcagcttc agcctatact taatctcgct cttcagggcg ccgtcctcgg ggtacatccg
      301 ctcctcggag gcctcccagc ccatggtctt cttctgcatt acggggccgt cggaggggaa
      361 gttggtgccg cgcagcttca ccttgtagat gaacacgccg tcctgcaggg aggagtcctg
      421 gttaacgtga ataatgccgc cgtcctcgaa gttcatcacg cgctcccacc tgaagccctc
      481 ggggaaggac agcttgaagt agtcggggat gtcggctggg tgcttaatgt agaccttgga
      541 gccgtacatg aactgagggg acaggatgtc ccaggcgaag ggcagggggc cacccttggt
      601 caccttcagc ttagcggtct gaaaggcctc gtaggggcgg ccctcgccct cgccctcgat
      661 ctcgaactcg tggccgttca cggagccctc catgtgcacc ttgaagcgca tgaactcctt
      721 gatgatggcc atgttattct cctcgccctt gctcaccatg accggtggat ccccaaactc
      781 attgtcaatg tcccaaacgt caccagagag ggcatttgca gctccctgaa tagtccaagt
      841 agctagagcc aactggtttc tgaacagcgt ttcattaaaa gcaccgttat tttgtttacg
      901 cagtttcaag ttctccagta aagctg
//
