LOCUS       sequence-101503-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human transferrin receptor tagged with pH sensitive orange/red 
            fluorescent protein pHoran4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Shen Y, Rosendale M, Campbell RE, Perrais D
  TITLE     pHuji, a pH-sensitive red fluorescent protein for imaging of exo- 
            and endocytosis.
  JOURNAL   J Cell Biol. 2014 Nov 10;207(3):419-32. doi: 10.1083/jcb.201404107.
  PUBMED    25385186
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2014-11-10- 10"; volume: "207"; 
            issue: "3"; pages: "419-32"
COMMENT     Sequence Label: TfR-pHoran4
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        233..251
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     233..250
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     264..283
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
       61 gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga acgcggattc
      121 cccgtgccaa gagtgacgta agtaccgcct atagagtcta taggcccacc cccttggctt
      181 cgttagaacg cggctacaat taatacataa ccttatgtat catacacata cgatttaggt
      241 gacactatag aataacatcc actttgcctt tctctccaca ggtgtccact cccaggtcca
      301 actgcacctc ggttctatcg attgaattcc ccggggatcc accatggatc aagctagatc
      361 agcattctct aacttgtttg gtggagaacc attgtcatat acccggttca gcctggctcg
      421 gcaagtagat ggcgataaca gtcatgtgga gatgaaactt gctgtagatg aagaagaaaa
      481 tgctgacaat aacacaaagg ccaatgtcac aaaaccaaaa aggtgtagtg gaagtatctg
      541 ctatgggact attgctgtga tcgtcttttt cttgattgga tttatgattg gctacttggg
      601 ctattgtaaa ggggtagaac caaaaactga gtgtgagaga ctggcaggaa ccgagtctcc
      661 agtgagggag gagccaggag aggacttccc tgcagcacgt cgcttatatt gggatgacct
      721 gaagagaaag ttgtcggaga aactggacag cacagacttc accagcacca tcaagctgct
      781 gaatgaaaat tcatatgtcc ctcgtgaggc tggatctcaa aaagatgaaa atcttgcgtt
      841 gtatgttgaa aat
//
