LOCUS       sequence-101505-         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Red fluorescent protein based pH indicator in pDisplay vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Shen Y, Rosendale M, Campbell RE, Perrais D
  TITLE     pHuji, a pH-sensitive red fluorescent protein for imaging of exo- 
            and endocytosis.
  JOURNAL   J Cell Biol. 2014 Nov 10;207(3):419-32. doi: 10.1083/jcb.201404107.
  PUBMED    25385186
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2014-11-10- 10"; volume: "207"; 
            issue: "3"; pages: "419-32"
COMMENT     Sequence Label: pDisplay-pHuji
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     146..205
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             209..235
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     670..689
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct
      121 ggaattcggc ttggggatat ccaccatgga gacagacaca ctcctgctat gggtactgct
      181 gctctgggtt ccaggttcca ctggtgacta tccatatgat gttccagatt atgctggggc
      241 ccagccggcc agatctatgg tgagcaaggg cgaggagaat aacatggcca tcatcaagga
      301 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
      361 gggcgagggc gagggccgcc cctacgaggc ctttcagacc gctaagctga aggtgaccaa
      421 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
      481 ggtctacatt aagcacccag ccgacatccc cgactacttc aagctgtcct tccccgaggg
      541 cttcaggtgg gagcgcgtga tgaacttcga ggacggcggc attattcacg ttaaccagga
      601 ctcctccctg caggacggcg tgttcatcta caaggtgaag ctgcgcggca ccaacttccc
      661 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctccg aggagcggat
      721 gtaccccgag gacggcgccc tgaagagcga gattaagtat aggctgaagc tgaaggacgg
      781 cggccactac gccgccgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
      841 cggcgcctac atcgtggaca tcaagttgga catcgtgtcc cac
//
