LOCUS       sequence-101508-         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Red fluorescent protein based pH indicator in pDisplay vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Shen Y, Rosendale M, Campbell RE, Perrais D
  TITLE     pHuji, a pH-sensitive red fluorescent protein for imaging of exo- 
            and endocytosis.
  JOURNAL   J Cell Biol. 2014 Nov 10;207(3):419-32. doi: 10.1083/jcb.201404107.
  PUBMED    25385186
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2014-11-10- 10"; volume: "207"; 
            issue: "3"; pages: "419-32"
COMMENT     Sequence Label: pDisplay-pHuji
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(133..162)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(172..879)
                     /codon_start=1
                     /product="pH-sensitive red fluorescent protein derived from
                     mApple, for imaging of exocytosis and endocytosis (Shen et 
                     al., 2014)"
                     /label=pHuji
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKYRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(447..466)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             complement(901..927)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 aagcatgatg aggatgataa gggagatgat ggtgagcacc accagggcca ggatggctga
       61 gatcaccacc accttaaagg gcaaggagtg tggcaccacg atgacctcct gcgtgtcctg
      121 gcccacagca ttcagatcct cttctgagat gagtttttgt tcgtcgacgc ccttgtacag
      181 ctcgtccatg ccgccggtgg agtggcggcc ctcggcgcgt tcgtactgtt ccacgatggt
      241 gtagtcctcg ttgtgggaca cgatgtccaa cttgatgtcc acgatgtagg cgccgggcag
      301 ctgcacgggc ttcttggcct tgtaggtggt cttgacctcg gcggcgtagt ggccgccgtc
      361 cttcagcttc agcctatact taatctcgct cttcagggcg ccgtcctcgg ggtacatccg
      421 ctcctcggag gcctcccagc ccatggtctt cttctgcatt acggggccgt cggaggggaa
      481 gttggtgccg cgcagcttca ccttgtagat gaacacgccg tcctgcaggg aggagtcctg
      541 gttaacgtga ataatgccgc cgtcctcgaa gttcatcacg cgctcccacc tgaagccctc
      601 ggggaaggac agcttgaagt agtcggggat gtcggctggg tgcttaatgt agaccttgga
      661 gccgtacatg aactgagggg acaggatgtc ccaggcgaag ggcagggggc cacccttggt
      721 caccttcagc ttagcggtct gaaaggcctc gtaggggcgg ccctcgccct cgccctcgat
      781 ctcgaactcg tggccgttca cggagccctc catgtgcacc ttgaagcgca tgaactcctt
      841 gatgatggcc atgttattct cctcgccctt gctcaccata gatctggccg gctgggcccc
      901 agcataatct ggaacatcat atggatagtc accagtgga
//
