LOCUS       sequence-101519-         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Orange fluorescent protein based pH indicator in pDisplay vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Shen Y, Rosendale M, Campbell RE, Perrais D
  TITLE     pHuji, a pH-sensitive red fluorescent protein for imaging of exo- 
            and endocytosis.
  JOURNAL   J Cell Biol. 2014 Nov 10;207(3):419-32. doi: 10.1083/jcb.201404107.
  PUBMED    25385186
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2014-11-10- 10"; volume: "207"; 
            issue: "3"; pages: "419-32"
COMMENT     Sequence Label: pDisplay-pHoran4
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     146..205
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     primer_bind     628..647
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttgg taccgaactc ggatccacta gtaacggccg ccagtgtgct
      121 ggaattcggc ttggggatat ccaccatgga gacagacaca ctcctgctat gggtactgct
      181 gctctgggtt ccaggttcca ctggtgacag atctatggtg agcaagggcg aggagaataa
      241 catggccatc atcaaggagt tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg
      301 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggct ttcagaccgc
      361 taagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
      421 gttcacctac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttcaa
      481 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
      541 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
      601 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
      661 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagaagag
      721 gctgaagctg aaggacggcg gccactacac ctccgaggtc aagaccacct acaaggccaa
      781 gaagcccgtg cagctgcccg gcgcctacat cgtcggcatc aagttggaca tcacctccca
      841 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
      901 cggcat
//
