LOCUS       sequence-10152-e        8212 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8212)
  AUTHORS   Kondo K, Kim WY, Lechpammer M, Kaelin WG
  TITLE     Inhibition of HIF2alpha is sufficient to suppress pVHL-defective 
            tumor growth.
  JOURNAL   PLoS Biol. 2003 Dec . 1(3):E83.
  PUBMED    14691554
REFERENCE   2  (bases 1 to 8212)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Biol. 
            2003 Dec . 1(3):E83."
COMMENT     Sequence Label: HA-HIF2-alpha MT-pBabe-Hygro
FEATURES             Location/Qualifiers
     source          1..8212
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             98..567
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    632..831
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1024..1440
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1408..1424
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1451..1477
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4132..4152)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4137..4466
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4317..4452
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4540..5565
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4540..5565
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             6360..6829
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(join(7314..8105,8106..8174))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7937..7956
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(8175..8212,1..67))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
       61 ttccgcgcac atttccccga aaagtgccac ctgcagcctg aatatgggcc aaacaggata
      121 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat
      181 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
      241 ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg
      301 tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct
      361 cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact
      421 cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct
      481 cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      541 tgactacccg tcagcggggg tctttcattt gggggctcgt ccgggatcgg gagacccctg
      601 cccagggacc accgacccac caccgggagg caagctggcc agcaacttat ctgtgtctgt
      661 ccgattgtct agtgtctatg actgatttta tgcgcctgcg tcggtactag ttagctaact
      721 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc
      781 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt
      841 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac
      901 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc
      961 tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat
     1021 tagggccaga ctgttaccac tcccttaagt ttgaccttag atcactggaa agatgtcgag
     1081 cggctcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct
     1141 gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc
     1201 atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc
     1261 ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt
     1321 gtacacccta agcctccgcc tcctcttctt ccatccgcgc cgtctctccc ccttgaacct
     1381 cctctttcga ccccgcctca atcctccctt tatccagccc tcactccttc tctaggcgcc
     1441 ggccaccatg tatccatatg atgttccaga ttatgctgga tccgccacca tgacagctga
     1501 caaggagaag aaaaggagta gctcggagag gaggaaggag aagtcccggg atgctgcgcg
     1561 gtgccggcgg agcaaggaga ccgaagtctt ctatgagctg gcccatgagc tgcctctgcc
     1621 ccacagtgtg agctcccatc tggacaaggc ctccatcatg cgactggcaa tcagcttcct
     1681 gcgaacacac aagctcctct cctcagtttg ctctgaaaac gagtccgaag ccgaagctga
     1741 ccagcagatg gacaacttgt acctgaaagc cttggagggt ttcattgccg tggtgaccca
     1801 agatggcgac atgatctttc tgtcagaaaa catcagcaag ttcatgggac ttacacaggt
     1861 ggagctaaca ggacatagta tctttgactt cactcatccc tgcgaccatg aggagattcg
     1921 tgagaacctg agtctcaaaa atggctctgg ttttgggaaa aaaagcaaag acatgtccac
     1981 agagcgggac ttcttcatga ggatgaagtg cacggtcacc aacagaggcc gtactgtcaa
     2041 cctcaagtca gccacctgga aggtcttgca ctgcacgggc caggtgaaag tctacaacaa
     2101 ctgccctcct cacaatagtc tgtgtggcta caaggagccc ctgctgtcct gcctcatcat
     2161 catgtgtgaa ccaatccagc acccatccca catggacatc cccctggata gcaagacctt
     2221 cctgagccgc cacagcatgg acatgaagtt cacctactgt gatgacagaa tcacagaact
     2281 gattggttac caccctgagg agctgcttgg ccgctcagcc tatgaattct accatgcgct
     2341 agactccgag aacatgacca agagtcacca gaacttgtgc accaagggtc aggtagtaag
     2401 tggccagtac cggatgctcg caaagcatgg gggctacgtg tggctggaga cccaggggac
     2461 ggtcatctac aaccctcgca acctgcagcc ccagtgcatc atgtgtgtca actacgtcct
     2521 gagtgagatt gagaagaatg acgtggtgtt ctccatggac cagactgaat ccctgttcaa
     2581 gccccacctg atggccatga acagcatctt tgatagcagt ggcaaggggg ctgtgtctga
     2641 gaagagtaac ttcctattca ccaagctaaa ggaggagccc gaggagctgg cccagctggc
     2701 tcccacccca ggagacgcca tcatctctct ggatttcggg aatcagaact tcgaggagtc
     2761 ctcagcctat ggcaaggcca tcctgccccc gagccagcca tgggccacgg agttgaggag
     2821 ccacagcacc cagagcgagg ctgggagcct gcctgccttc accgtgcccc aggcagctgc
     2881 cccgggcagc accaccccca gtgccaccag cagcagcagc agctgctcca cgcccaatag
     2941 ccctgaagac tattacacat ctttggataa cgacctgaag attgaagtga ttgagaagct
     3001 cttcgccatg gacacagagg ccaaggacca atgcagtacc cagacggatt tcaatgagct
     3061 ggacttggag acactggcac cctatatccc catggacggg gaagacttcc agctaagccc
     3121 catctgcccc gaggagcggc tcttggcgga gaacccacag tccacccccc agcactgctt
     3181 cagtgccatg acaaacatct tccagccact ggcccctgta gccccgcaca gtcccttcct
     3241 cctggacaag tttcagcagc agctggagag caagaagaca gagcccgagc accggcccat
     3301 gtcctccatc ttctttgatg ccggaagcaa agcatccctg ccaccgtgct gtggccaggc
     3361 cagcacccct ctctcttcca tggggggcag atccaatacc cagtggcccc cagatccacc
     3421 attacatttt gggcccacaa agtgggccgt cggggatcag cgcacagagt tcttgggagc
     3481 agcgccgttg gggccccctg tctctccacc ccatgtctcc accttcaaga caaggtctgc
     3541 aaagggtttt ggggctcgag gcccagacgt gctgagtccg gccatggtag ccctctccaa
     3601 caagctgaag ctgaagcgac agctggagta tgaagagcaa gccttccagg acctgagcgg
     3661 gggggaccca cctggtggca gcacctcaca tttgatgtgg aaacggatga agaacctcag
     3721 gggtgggagc tgccctttga tgccggacaa gccactgagc gcaaatgtac ccaatgataa
     3781 gttcacccaa aaccccatga ggggcctggg ccatcccctg agacatctgc cgctgccaca
     3841 gcctccatct gccatcagtc ccggggagaa cagcaagagc aggttccccc cacagtgcta
     3901 cgccacccag taccaggact acagcctgtc gtcagcccac aaggtgtcag gcatggcaag
     3961 ccggctgctc gggccctcat ttgagtccta cctgctgccc gaactgacca gatatgactg
     4021 tgaggtgaac gtgcccgtgc tgggaagctc cacgctcctg caaggagggg acctcctcag
     4081 agccctggac caggccacct gacaattcgc cagcacagtg gtcgaccctg tggaatgtgt
     4141 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     4201 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     4261 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     4321 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     4381 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     4441 tttttggagg cctaggcttt tgcaaaaagc ttgccagctg gggcgccctc tggtaaggtt
     4501 gggaagccct gcagatctga tcccgggggg caatgagata tgaaaaagcc tgaactcacc
     4561 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag
     4621 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc
     4681 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt
     4741 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaatttag cgagagcctg
     4801 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa
     4861 ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt
     4921 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg
     4981 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac
     5041 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac
     5101 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac
     5161 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac
     5221 gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc
     5281 tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc
     5341 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct
     5401 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca
     5461 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat
     5521 agtggaaacc gacgccccag cactcgtccg agggcaaagg aatagagtag atgccgaccg
     5581 ggatctatcg atgcggcggg tgtggtggtt acgcgtaatc tgctgcttgc aaacaaaaaa
     5641 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5701 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     5761 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     5821 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     5881 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     5941 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6001 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6061 gcgcacgagg gagcttccag gggaaacgcc tggtatctta tagtcctgtc gggtttcgcc
     6121 acctctgact tgagcgtcga ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6181 cgccagcaac gcatcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat
     6241 gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg
     6301 gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc
     6361 tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag
     6421 aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc
     6481 cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga
     6541 gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga
     6601 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata
     6661 aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt
     6721 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt
     6781 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcac atgcagcatg
     6841 tatcaaaatt aatttggttt tttttcttaa gtatttacat taaatggcca tagtgcggcc
     6901 gcaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     6961 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7021 tcgtgtagat aactacgata cgggagggct taccatctgg ccccccaagg cggtaatacg
     7081 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7141 ggccaggaac cgtaaaaagg ccgcgttacg ctcagtggaa cgaaaactca cgttaaggga
     7201 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7261 gtttgcggcc gcaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     7321 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     7381 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     7441 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     7501 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     7561 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     7621 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     7681 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     7741 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     7801 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     7861 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     7921 ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     7981 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     8041 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     8101 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     8161 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gg
//
