LOCUS       sequence-101520-         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Orange fluorescent protein based pH indicator in pDisplay vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Shen Y, Rosendale M, Campbell RE, Perrais D
  TITLE     pHuji, a pH-sensitive red fluorescent protein for imaging of exo- 
            and endocytosis.
  JOURNAL   J Cell Biol. 2014 Nov 10;207(3):419-32. doi: 10.1083/jcb.201404107.
  PUBMED    25385186
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2014-11-10- 10"; volume: "207"; 
            issue: "3"; pages: "419-32"
COMMENT     Sequence Label: pDisplay-pHoran4
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(144..173)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(183..890)
                     /codon_start=1
                     /product="orange monomeric derivative of DsRed fluorescent 
                     protein (Shanet et al., 2004)"
                     /label=mOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKKRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(458..477)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     sig_peptide     complement(900..959)
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
ORIGIN
        1 ttcttctgcc aaagcatgat gaggatgata agggagatga tggtgagcac caccagggcc
       61 aggatggctg agatcaccac caccttaaag ggcaaggagt gtggcaccac gatgacctcc
      121 tgcgtgtcct ggcccacagc attcagatcc tcttctgaga tgagtttttg ttcgtcgacg
      181 cccttgtaca gctcgtccat gccgccggtg gagtggcggc cctcggcgcg ttcgtactgt
      241 tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca acttgatgcc gacgatgtag
      301 gcgccgggca gctgcacggg cttcttggcc ttgtaggtgg tcttgacctc ggaggtgtag
      361 tggccgccgt ccttcagctt cagcctcttc ttgatctcgc ccttcagggc gccgtcctcg
      421 gggtacatcc gctcggagga ggcctcccag cccatggtct tcttctgcat tacggggccg
      481 tcggagggga agttggtgcc gcgcagcttc accttgtaga tgaactcgcc gtcctgcagg
      541 gaggagtcct gggtcacggt caccacgccg ccgtcctcga agttcatcac gcgctcccac
      601 ttgaagccct cggggaagga cagcttgaag tagtcgggga tgtcggcggg gtgcttcacg
      661 taggccttgg agccgtaggt gaactgaggg gacaggatgt cccaggcgaa gggcaggggg
      721 ccacccttgg tcaccttcag cttagcggtc tgaaagccct cgtaggggcg gccctcgccc
      781 tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgcgcac cttgaagcgc
      841 atgaactcct tgatgatggc catgttattc tcctcgccct tgctcaccat agatctgtca
      901 ccagtggaac ctggaaccca gagcagcagt acccatagca ggagtgtgtc tgtctccatg
      961 gtggatatc
//
