LOCUS       sequence-101524-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Red fluorescent protein based pH indicator in bacterial expression 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Shen Y, Rosendale M, Campbell RE, Perrais D
  TITLE     pHuji, a pH-sensitive red fluorescent protein for imaging of exo- 
            and endocytosis.
  JOURNAL   J Cell Biol. 2014 Nov 10;207(3):419-32. doi: 10.1083/jcb.201404107.
  PUBMED    25385186
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2014-11-10- 10"; volume: "207"; 
            issue: "3"; pages: "419-32"
COMMENT     Sequence Label: pBAD-pHuji
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             66..83
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             87..119
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(123..131,132..146)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     575..594
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 actctctact gtttctccat acccgttttt tgggctaaca ggaggaatta accatggggg
       61 gttctcatca tcatcatcat catggtatgg ctagcatgac tggtggacag caaatgggtc
      121 gggatctgta cgacgatgac gataaggatc cgagctcgag aatggtgagc aagggcgagg
      181 agaataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
      241 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gaggcctttc
      301 agaccgctaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
      361 cccctcagtt catgtacggc tccaaggtct acattaagca cccagccgac atccccgact
      421 acttcaagct gtccttcccc gagggcttca ggtgggagcg cgtgatgaac ttcgaggacg
      481 gcggcattat tcacgttaac caggactcct ccctgcagga cggcgtgttc atctacaagg
      541 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
      601 gctgggaggc ctccgaggag cggatgtacc ccgaggacgg cgccctgaag agcgagatta
      661 agtataggct gaagctgaag gacggcggcc actacgccgc cgaggtcaag accacctaca
      721 aggccaagaa gcccgtgcag ctgcccggcg cctacatcgt ggacatcaag ttggacatcg
      781 tgtcccacaa cgaggactac
//
