LOCUS       sequence-101532-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PK1F GFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Segall-Shapiro TH, Meyer AJ, Ellington AD, Sontag ED, Voigt CA
  TITLE     A 'resource allocator' for transcription based on a highly 
            fragmented T7 RNA polymerase.
  JOURNAL   Mol Syst Biol. 2014 Jul 30;10(7):742.
  PUBMED    25080493
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-07-30"; volume: "10(7)"; pages: "742"
COMMENT     Sequence Label: pTHSSd_10
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(89..107)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             505..883
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(539..558)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctttgctcat ctttacctcc tctatcgcgg aaattgacag gatcctctcc ctatagtgat
       61 agttattact cgaggtgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
      121 gtcatgataa taatggtttc ttagacgtcg gaattgccag ctggggcgcc ctctggtaag
      181 gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat ctgatggcgc
      241 aggggatcaa gatctgatca agagacagga tgaggatcgt ttcgcgataa atgcttcaat
      301 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      361 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      421 ctgaagatca gttgggtgca cgagtgggtt atatcgaact ggatctcaac agcggtaaga
      481 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      541 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      601 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      661 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      721 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      781 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      841 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      901 gcgaactact tactctagct tcccgg
//
