LOCUS       sequence-101539-         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  High full length T7 RNAP* expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Segall-Shapiro TH, Meyer AJ, Ellington AD, Sontag ED, Voigt CA
  TITLE     A 'resource allocator' for transcription based on a highly 
            fragmented T7 RNA polymerase.
  JOURNAL   Mol Syst Biol. 2014 Jul 30;10(7):742.
  PUBMED    25080493
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-07-30"; volume: "10(7)"; pages: "742"
COMMENT     Sequence Label: pTHSSd_41
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        137..171
                     /label=BBa_J23105
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.24"
ORIGIN
        1 cacttgtatt gtcgatcaga ctatcagcgt gagactacga ttccatcaat gcctgtcaag
       61 ggcaagtatt gacatgtcgt cgtaacctgt agaacggagt aacctcggtg tgcggttgta
      121 tgcctgctgt ggattgttta cggctagctc agtcctaggt actatgctag ctactagagt
      181 catttatgaa agtactagat gaacacgatt aacatcgcta agaacgactt ctctgacatc
      241 gaactggctg ctatcccgtt caacactctg gctgaccatt acggtgagcg tttagctcgc
      301 gaacagttgg cccttgagca tgagtcttac gagatgggtg aagcacgctt ccgcaagatg
      361 tttgagcgtc aacttaaagc tggtgaggtt gcggataacg ctgccgccaa gcctctcatc
      421 actaccctac tccctaagat gattgcacgc atcaacgact ggtttgagga agtgaaagct
      481 aagcgcggca agcgcccgac agccttccag ttcctgcaag aaatcaagcc ggaagccgta
      541 gcgtacatca ccattaagac cactctggct tgcctaacca gtgctgacaa tacaaccgtt
      601 caggctgtag caagcgcaat cggtcgggcc attgaggacg aggctcgctt cggtcgtatc
      661 cgtgaccttg aagctaagca cttcaagaaa aacgttgagg aacaactcaa caagcgcgta
      721 gggcacgtct acaagaaagc atttatgcaa gttgtcgagg ctgacatgct ctctaagggt
      781 ctactcggtg gcgaggcgtg gtcttcgtgg cataaggaag actctattca tgtaggagta
      841 cgctgcatcg agatgctcat tg
//
