LOCUS       sequence-101545-         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral vector for LMO2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Elcheva I, Brok-Volchanskaya V, Kumar A, Liu P, Lee JH, Tong L, 
            Vodyanik M, Swanson S, Stewart R, Kyba M, Yakubov E, Cooke J, 
            Thomson JA, Slukvin I
  TITLE     Direct induction of haematoendothelial programs in human pluripotent
            stem cells by transcriptional regulators.
  JOURNAL   Nat Commun. 2014 Jul 14;5:4372. doi: 10.1038/ncomms5372.
  PUBMED    25019369
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-07-14"; volume: "5:4372. doi"; pages: " 
            10.1038/ncomms5372"
COMMENT     Sequence Label: pSIN4-EF1a-LMO2-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      85..94
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             94..117
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(705..728)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ttttgacctc catagaagac accgactcta ctagaggatc gctagcgcta ccggactcag
       61 atctcgagct caagcttcga attcgccacc atggactaca aggacgacga tgacaagtcc
      121 tcggccatcg aaaggaagag cctggaccct tcagaggaac cagtggatga ggtgctgcag
      181 atccccccat ccctgctgac atgcggcggc tgccagcaga acattgggga ccgctacttc
      241 ctgaaggcca tcgaccagta ctggcacgag gactgcctga gctgcgacct ctgtggctgc
      301 cggctgggtg aggtggggcg gcgcctctac tacaaactgg gccggaagct ctgccggaga
      361 gactatctca ggctttttgg gcaagacggt ctctgcgcat cctgtgacaa gcggattcgt
      421 gcctatgaga tgacaatgcg ggtgaaagac aaagtgtatc acctggaatg tttcaagtgc
      481 gccgcctgtc agaagcattt ctgtgtaggt gacagatacc tcctcatcaa ctctgacata
      541 gtgtgcgaac aggacatcta cgagtggact aagatcaatg ggatgatata gacgcgtcgg
      601 aatcactagt gaattctgca gtcgacggta ccgcgggccc gggatcccgc gactctagat
      661 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
      721 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
      781 ccggtaggcg
//
