LOCUS       sequence-101554-         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to express human AID recoded for yeast codon usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Mayorov VI, Rogozin IB, Adkison LR, Frahm C, Kunkel TA, Pavlov YI
  TITLE     Expression of human AID in yeast induces mutations in context 
            similar to the context of somatic hypermutation at G-C pairs in 
            immunoglobulin genes.
  JOURNAL   BMC Immunol. 2005 Jun 10;6:10.
  PUBMED    15949042
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Immunol."; date: "2005-06-10"; volume: "6"; pages: "10"
COMMENT     Sequence Label: pESC-LEU-HsAIDSc
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(130..295)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     216..240
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(443..466)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(516..846)
                     /gene="S. cerevisiae GAL10"
                     /label=GAL10 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    729..846
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tccattcgcc
       61 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
      121 gctgaattgg agcgacctca tgctatacct gagaaagcaa cctgacctac aggaaagagt
      181 tactcaagaa taagaatttt cgttttaaaa cctaagagtc actttaaaat ttgtatacac
      241 ttattttttt tataacttat ttaataataa aaatcataaa tcataagaaa ttcgcttatt
      301 tagaagtgtc aacaacgtat ctaccaacga tttgaccctt ttccatcttt tcgtaaattt
      361 ctggcaaggt agacaagccg acaaccttga ttggagactt gaccaaacct ctggcgaaga
      421 attgttaatt aagagctcag atcttatcgt cgtcatcctt gtaatccatc gatactagtg
      481 cggccgccct ttagtgaggg ttgaattcga attttcaaaa attcttactt tttttttgga
      541 tggacgcaaa gaagtttaat aatcatatta catggcatta ccaccatata catatccata
      601 tacatatcca tatctaatct tacttatatg ttgtggaaat gtaaagagcc ccattatctt
      661 agcctaaaaa aaccttctct ttggaacttt cagtaatacg cttaactgct cattgctata
      721 ttgaagtacg gattagaagc cgccgagcgg gtgacagccc tccgaaggaa gactctcctc
      781 cgtgcgtcct cgtcttcacc ggtcgcgttc ctgaaacgca gatgtgcctc gcgccgcact
      841 gctccgaaca ataaagattc tacaatacta gcttttatgg ttatgaagag gaaaaattgg
      901 cagtaacctg gccccacaaa ccttcaaatg aacgaatcaa attaacaacc ataggatgat
      961 aatgcgatta gtt
//
