LOCUS       sequence-101555-        1125 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to express human AID recoded for yeast codon usage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Mayorov VI, Rogozin IB, Adkison LR, Frahm C, Kunkel TA, Pavlov YI
  TITLE     Expression of human AID in yeast induces mutations in context 
            similar to the context of somatic hypermutation at G-C pairs in 
            immunoglobulin genes.
  JOURNAL   BMC Immunol. 2005 Jun 10;6:10.
  PUBMED    15949042
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Immunol."; date: "2005-06-10"; volume: "6"; pages: "10"
COMMENT     Sequence Label: pESC-LEU-HsAIDSc
FEATURES             Location/Qualifiers
     source          1..1125
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     216..233
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(305..494)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(524..553)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
       61 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      121 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      181 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      241 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      301 ggatcttcga gcgtcccaaa accttctcaa gcaaggtttt cagtataatg ttacatgcgt
      361 acacgcgtct gtacagaaaa aaaagaaaaa tttgaaatat aaataacgtt cttaatacta
      421 acataactat aaaaaaataa atagggacct agacttcagg ttgtctaact ccttcctttt
      481 cggttagagc ggatcttagc tagccgcggt accaagctta ctcgaggtct tcttcggaaa
      541 tcaacttctg ttccatgtcg actaaaccca aggttctgaa agcatctctc aaatcatcga
      601 cttcatataa tggtaacaaa attcttctca attgacggct caatctgacg gagttctcgt
      661 gcaaaccttc ccaagccttg aaggttcttt catggttttc aacgaaagtg ttccagcagt
      721 agaagtagtc cttgaaggtc atgatggcaa tttgaacacc agctctgtgc aatcttctca
      781 aaccttctgg ttcagccttt ctgtcttcac agaagtacaa acgagcggtg aagattctca
      841 aggacaagtt tgggttacct ctcaagaagt cggcaacgtg tctggcacag tcgtaacaag
      901 gggaccaaga agtgaaccaa gtaactctgt aacatctacc tgggtccaag tcccaatcag
      961 agatgtatct caagaatagc aattcgacgt gacaaccgtt tttgtttctc aagtaaccga
     1021 agtccaagga gaaagaagta gcagagtctc ttctcttaac gacgtaacac aagtaggttt
     1081 ctcttctacc cttagcccat ctgacgttct taaattggta caaaa
//
