LOCUS       sequence-101559-        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to overexpress yeast polymerase eta in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Pavlov YI, Nguyen D, Kunkel TA
  TITLE     Mutator effects of overproducing DNA polymerase eta (Rad30) and its 
            catalytically inactive variant in yeast.
  JOURNAL   Mutat Res. 2001 Jul 1;478(1-2):129-39.
  PUBMED    11406177
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mutat 
            Res."; date: "2001-07-1"; volume: "478(1-2)"; pages: "129-39"
COMMENT     Sequence Label: pESC-LEU2-RAD30cmyc
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(143..308)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     229..253
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(456..479)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(529..859)
                     /gene="S. cerevisiae GAL10"
                     /label=GAL10 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    742..859
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
       61 gcgtccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
      121 cgctattacg ccagctgaat tggagcgacc tcatgctata cctgagaaag caacctgacc
      181 tacaggaaag agttactcaa gaataagaat tttcgtttta aaacctaaga gtcactttaa
      241 aatttgtata cacttatttt ttttataact tatttaataa taaaaatcat aaatcataag
      301 aaattcgctt atttagaagt gtcaacaacg tatctaccaa cgatttgacc cttttccatc
      361 ttttcgtaaa tttctggcaa ggtagacaag ccgacaacct tgattggaga cttgaccaaa
      421 cctctggcga agaattgtta attaagagct cagatcttat cgtcgtcatc cttgtaatcc
      481 atcgatacta gtgcggccgc cctttagtga gggttgaatt cgaattttca aaaattctta
      541 cttttttttt ggatggacgc aaagaagttt aataatcata ttacatggca ttaccaccat
      601 atacatatcc atatacatat ccatatctaa tcttacttat atgttgtgga aatgtaaaga
      661 gccccattat cttagcctaa aaaaaccttc tctttggaac tttcagtaat acgcttaact
      721 gctcattgct atattgaagt acggattaga agccgccgag cgggtgacag ccctccgaag
      781 gaagactctc ctccgtgcgt cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc
      841 ctcgcgccgc actgctccga acaataaaga ttctacaata ctagctttta tggttatgaa
      901 gaggaaaaat tggcagtaac ctggccccac aaaccttcaa atgaacgaat caaattaaca
      961 accataggat gataatgcga ttagtttttt agccttattt ctggggtaat taatcagcga
     1021 agcgatgatt tttgatctat taacagatat ataaatgcaa aa
//
