LOCUS       sequence-101561-        6486 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses firefly luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6486)
  AUTHORS   Chang M, Anttonen KP, Cirillo SL, Francis KP, Cirillo JD
  TITLE     Real-time bioluminescence imaging of mixed mycobacterial infections.
  JOURNAL   PLoS One. 2014 Sep 29;9(9):e108341. doi: 
            10.1371/journal.pone.0108341. eCollection 2014.
  PUBMED    25265287
REFERENCE   2  (bases 1 to 6486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-09-29"; volume: "9(9):e108341. doi"; pages: " 
            10.1371/journal.pone.0108341. eCollection 2014"
COMMENT     Sequence Label: pJDC182
FEATURES             Location/Qualifiers
     source          1..6486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(4407..4995)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4487..4506)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(6365..6411)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 taacggccgc cagtgtgctg gaattcggct tttggtcgat accaagccat ttccgctgaa
       61 tatcgtggag ctcaccgcca gaatcggtgg ttgtggtgat gtacgtggcg aactccgttg
      121 tagtgcttgt ggtggcatcc gtggcgcggc cgcggtacca gatctttaaa tctagaggtg
      181 accacaacga cgcgcccgct ttgatcgggg acgtctgcgg ccgaccattt acgggtcttg
      241 ttgtcgttgg cggtcatggg ccgaacatac tcacccggat cggagggccg aggacaaggt
      301 cgaacgaggg gcatgacccg gtgcggggct tcttgcactc ggcataggcg agtgctaaga
      361 ataacgttgg cactcgcgac cggtgagtcg taggtcggga cggtgaggcc aggcccgtcg
      421 tcgcagcgag tggcagcgag gacaacttga gccgtccgtc gcgggcactg cgcccggcca
      481 gcgtaagtag cggggttgcc gtcacccggt gacccccggt ttcatccccg atccggagga
      541 atcacttcgc aatggccaag acaattgcgg atccagctgc aggctagcag gaggataaca
      601 tatggaagat gcgaagaaca tcaagaaagg cccagcgccg ttctacccgc tggaggacgg
      661 caccgccggt gaacagctgc acaaggcgat gaaacgctac gcgttggtcc ccggcaccat
      721 cgccttcacc gatgcgcaca tcgaggtcaa catcacctac gcggaatact tcgagatgtc
      781 cgtgcgcctg gccgaggcca tgaagcgcta cgggctgaac accaatcatc gcatcgtggt
      841 gtgtagcgaa aattcgctgc agttcttcat gccggtgctg ggcgcgttgt tcatcggcgt
      901 ggcggtggcc ccggcgaacg acatctacaa cgaacgcgag ctgctgaact ccatgaacat
      961 ctcgcaaccg acggtggtgt tcgtgtcgaa gaaagggctg cagaagatcc tgaacgtcca
     1021 gaagaagctc ccaatcattc agaagatcat tatcatggac agcaagacgg attatcaggg
     1081 cttccagtcc atgtacacct tcgtcacgtc ccacttgccg ccgggcttta atgagtatga
     1141 tttcgtgcca gaatcgttcg atcgtgacaa gaccatcgcg ttgatcatga actcgtcggg
     1201 ctccaccggc ctgccgaagg gcgtggccct cccgcaccgg accgcgtgcg tccgcttctc
     1261 gcacgcccgg gacccaatct ttggcaatca gattatcccg gacacggcca tcttgagcgt
     1321 cgtcccgttt catcacggtt tcggcatgtt caccaccttg ggctacctga tctgtggctt
     1381 ccgggtcgtg ctcatgtacc gtttcgagga agagctgttc ttgcgtagcc tgcaagacta
     1441 caagatccaa agcgccctgt tggtcccgac gctgttctcg ttcttcgcga aaagcacctt
     1501 gatcgacaag tacgacttgt cgaatctgca cgagatcgcg tcgggtggtg cgcccctgag
     1561 caaagaggtg ggcgaggccg tggcgaagcg gttccacctc ccgggtatcc gccagggata
     1621 cggcctgacc gagacgacgt ccgcgatcct catcaccccg gagggcgacg acaagccagg
     1681 tgccgtgggc aaggtcgtgc cgtttttcga agcgaaggtc gtggacctgg acaccggaaa
     1741 gacgctgggc gtgaaccagc gcggggaact gtgcgtgcgc ggtccgatga tcatgtccgg
     1801 ttatgtgaat aacccggagg cgaccaatgc gctcatcgat aaagatggct ggttgcactc
     1861 gggcgacatc gcctactggg acgaagatga gcatttcttc atcgtggacc gtttgaagag
     1921 cctcatcaag tataagggtt accaggtggc cccagcggaa ttggaaagca ttctgctcca
     1981 gcacccgaac atcttcgacg cgggagtcgc cggcctcccg gacgatgatg ccggcgaact
     2041 gccagcggcc gtggtcgtcc tggagcacgg aaagacgatg accgaaaagg agatcgtgga
     2101 ctacgtcgcc tcgcaggtga ccaccgcgaa aaagctgcgt ggcggggtcg tctttgtgga
     2161 cgaagtgccg aaagggttga ccggaaagtt ggacgcccgg aagatccgtg aaatcttgat
     2221 caaagcgaag aaaggaggca agtcgaagct gtgattaatt aagcatgcgg ccggccagta
     2281 ctctgcagct ggatccatgg atatctagat ttaaagatct ggtaccgcgg ccgcgccacg
     2341 gatgccacca caggcactac aacggagttc gccacgtaca tcaccacaac caccgattct
     2401 ggcggtgagc tccccgatat tcagcggaaa tggcttggta tcgaccaaga ttcgtagaac
     2461 cccgtctcgt ctggctggta ttcaaaacgg gcgcaacgaa acacgcaacg agacaggcat
     2521 ggcccaaacc agaaaactag cgtctaccag gacttttacc tgtccgaccc gttgcaacgg
     2581 aaccccccac ggaacccccg cgacacccgc tccccaattg cgttagaaca gcggtggatt
     2641 gtcggcttcg ttgtgggcct tttgagccgc ttcctgttct gccgcacgct ctttcctcgc
     2701 ccgatagccg agtcgcttaa cggtgtccag atgcagcccg aaatgtttgg ccgtttgcgg
     2761 ccaagagtgg ccctcgtcgt cgtgataggc gcggatgcgt tcgcggcgtg cagcctgctc
     2821 ggcgagccac tcgctgcgtt cctgcgccac gagccggacg acgtggcgtt cggatagtcc
     2881 ggtgattcga gcgccttcgg cggcggtcac gcgccgcttt ttgcggacag tcggctgccg
     2941 gttgtagccg tcgctgtagc cgtcgctgta gccgtcgctc atagcaatgc ctccatgcat
     3001 ggctgacgcg gactttgcgc gccgcgcaac tgtgctcgcc gccgtgcgcg ctgctgcgcc
     3061 cttccgcgag atggccgact ggcgcgcact gagtgtggcc tcgtagacca cgatcccgtc
     3121 cgcccaaatg cgcgacttgg ttgtgatcca acgccaaatg ctgttggcga tggcgcggac
     3181 ctcgctgtcc ggtagcggtc cgggacacac gtcgttgcac ggaaattcgg cgtttcgcgc
     3241 gtggcactcg gcatagatcg cgcggccgag tccgtccacg ttccgggtcg gcaggtagat
     3301 ccgcatgagg gcgggacgat aggcccacaa cctgacggaa tcgaacagtg cgcaattccg
     3361 ccctagcggc gtcggagccg ctttgtacgt ggtctgctga cgccagcgcg gcggtggcat
     3421 gttcgcgccg agctcggcct cgatgtggct gagtgtgtag agatctgagt ggagccattc
     3481 cgtttcccag gcgatgtggc cggggttttt ggtcatgagg cctgagtaac tgcggtcgcc
     3541 atcgacggcg cgccgaaggc cttcggcgca cgccgccatg tatgcgagcg gcttacgccg
     3601 cgcgtattcg gtgcgtggaa caggggcgtt gagtgcccac actgcgtgtg cgtggccgtt
     3661 ggcgcgattg cccacgatcg cgttgggcag cggatgggac ccccgggcgc tgagcgctcg
     3721 gagcgctgcg tctggatggt ctacgtccac gaccagcagg tttgccagcg ctgttgggtt
     3781 cgcctcgatg taccggcggc ctagggccga cgcgcggctt tggcggtaga tcccctcgag
     3841 cagatcgtcg cttgccagcg gccagtacgg cagccagagc tgctcaaatt cgtcggcgac
     3901 gtggctcacg cttggtagta gaccacgatt aatcaccggt gtatggtccg acacgagctc
     3961 caagtcagat atttcgctga ggggccaccc cacaactgca cactcccccg ctctcccgtc
     4021 gagccctggt ggtggaacac cagcgacagc cgagcacccc caaccacctg taccaaccag
     4081 gaggaacaca tgcgtcgttt cgaggacgtt tccgggccgc tgagagccgc tgtggcggcc
     4141 gtacacgccg ccttagaccc gttagacccc ctgccgcctg aatgcgcggg tacgagccac
     4201 acagcgcccg aacttacgga gctggtgggc tcacgcgtgc ggccgcacgc gttgcgctcg
     4261 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     4321 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     4381 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     4441 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     4501 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     4561 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
     4621 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     4681 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     4741 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     4801 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     4861 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     4921 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     4981 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     5041 tagagcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     5101 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     5161 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     5221 gacgtggccg accagcccgt catcgtcaac gcctgaccgc ggtgcggaca ggccgtgtcg
     5281 cgaccggccg tgcggaatta agccggcccg taccctgtga atagaggtcc gctgtgacac
     5341 aagaatccct gttacttctc gaccgtattg attcggatga ttcctacgcg agcctgcgga
     5401 acgaccagga attctgggag ccgctggccc gccgagccct ggaggagctc gggctgccgg
     5461 tgccgccggt gctgcgggtg cccggcgaga gcaccaaccc cgtactggtc ggcgagcccg
     5521 acccggtgat caagctgttc ggcgagcact ggtgcggtcc ggagagcctc gcgtcggagt
     5581 cggaggcgta cgcggtcctg gcggacgccc cggtgccggt gccccgcctc ctcggccgcg
     5641 gcgagctgcg gcccggcacc ggagcctggc cgtggcccta cctggtgatg agccggatga
     5701 ccggcaccac ctggcggtcc gcgatggacg gcacgaccga ccggaacgcg ctgctcgccc
     5761 tggcccgcga actcggccgg gtgctcggcc ggctgcacag ggtgccgctg accgggaaca
     5821 ccgtgctcac cccccattcc gaggtcttcc cgaactgctg cgggacgccg cgcggcgccg
     5881 ctgaggacca ccgcgggtgg ggctacctct ggccccggct gctggaccgc ctggaggact
     5941 ggctgccgga cgtggacacg ctgctggccg gccgcgaacc ccggttcgtc cacggcgacc
     6001 tgcacgggac caacatcttc gtggacctgg ccgcgaccga ggtcaccggg atcgtcgact
     6061 tcaccgacgt ctatgcggga gactcccgct acagcctggt gcaactgcat ctcaacgcct
     6121 tccggggcga ccgcgagatc ctggccgcgc tgctcgacgg ggcgcagtgg aagcggaccg
     6181 aggacttcgc ccgcgaactg ctcgccttca ccttcctgca cgacttcgag gtgttcgagg
     6241 agaccccgct ggatctctcc ggcttcaccg atccggagga actggcgcag ttcctctggg
     6301 ggccgccgga caccgccccc ggcgcctgac gccccctcta gctgatcacc gcggccatga
     6361 tggcataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcctggc
     6421 agtcgatcgt acgctagtta actacgtcga catcgataag cttggtaccg agctcggatc
     6481 cactag
//
