LOCUS       sequence-101566-        1166 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector under the neuron-specific promoter 
            NeuroD. Co-expression of GFP with IRES sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Guerrier S, Coutinho-Budd J, Sassa T, Gresset A, Jordan NV, Chen K, 
            Jin WL, Frost A, Polleux F
  TITLE     The F-BAR domain of srGAP2 induces membrane protrusions required for
            neuronal migration and morphogenesis.
  JOURNAL   Cell. 2009 Sep 4;138(5):990-1004. doi: 10.1016/j.cell.2009.06.047.
  PUBMED    19737524
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2009.06"; journalName: "Cell"; date: "2009-09-4- 4";
            volume: "138"; issue: "5"; pages: "990-1004"
COMMENT     Sequence Label: pNeuroD-ires-GFP
FEATURES             Location/Qualifiers
     source          1..1166
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(165..167,168..170,171..884)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(165..167,168..170,171..881)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(210..231)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(471..490)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     818..839
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1007..1128
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1044..1063)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1098..1117
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtacac
       61 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga
      121 cgtggttttc ctttgaaaaa cacgatgata atatggccac aaccatggtg agcaagggcg
      181 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      241 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
      301 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
      361 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
      421 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
      481 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
      541 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
      601 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
      661 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
      721 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
      781 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
      841 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagcggc cgcgactcta
      901 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
      961 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     1021 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     1081 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg
     1141 taagcgttaa tattttgtta aaattc
//
