LOCUS       sequence-101567-        6460 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses click beetle red luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Chang M, Anttonen KP, Cirillo SL, Francis KP, Cirillo JD
  TITLE     Real-time bioluminescence imaging of mixed mycobacterial infections.
  JOURNAL   PLoS One. 2014 Sep 29;9(9):e108341. doi: 
            10.1371/journal.pone.0108341. eCollection 2014.
  PUBMED    25265287
REFERENCE   2  (bases 1 to 6460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-09-29"; volume: "9(9):e108341. doi"; pages: " 
            10.1371/journal.pone.0108341. eCollection 2014"
COMMENT     Sequence Label: pJDC181
FEATURES             Location/Qualifiers
     source          1..6460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(4381..4969)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4461..4480)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(6339..6385)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 taacggccgc cagtgtgctg gaattcggct tttggtcgat accaagccat ttccgctgaa
       61 tatcgtggag ctcaccgcca gaatcggtgg ttgtggtgat gtacgtggcg aactccgttg
      121 tagtgcttgt ggtggcatcc gtggcgcggc cgcggtacca gatctttaaa tctagaggtg
      181 accacaacga cgcgcccgct ttgatcgggg acgtctgcgg ccgaccattt acgggtcttg
      241 ttgtcgttgg cggtcatggg ccgaacatac tcacccggat cggagggccg aggacaaggt
      301 cgaacgaggg gcatgacccg gtgcggggct tcttgcactc ggcataggcg agtgctaaga
      361 ataacgttgg cactcgcgac cggtgagtcg taggtcggga cggtgaggcc aggcccgtcg
      421 tcgcagcgag tggcagcgag gacaacttga gccgtccgtc gcgggcactg cgcccggcca
      481 gcgtaagtag cggggttgcc gtcacccggt gacccccgtt tcatccccga tccggaggaa
      541 tcacttcgca atggccaaga caattgcgga tccagctgca ggctagcagg agggaattca
      601 tggtcaagcg cgagaagaac gtgatctacg gcccggagcc gctgcatccg ctggaggacc
      661 tgacggccgg cgagatgctg ttccgcgcgc tccgcaagca ctcgcacctg ccgcaagccc
      721 tggtggacgt cgtgggcgac gagtccctgt cgtacaagga gttcttcgag gcgacggtgc
      781 tgctggcgca atcgctgcat aactgcggct acaagatgaa cgacgtggtg tccatctgtg
      841 cggaaaacaa cacccgcttc ttcatccccg tcatcgccgc ctggtacatc ggcatgatcg
      901 tcgcgcccgt gaacgaatcc tacatccccg acgagctgtg taaggtgatg ggtatctcca
      961 agccgcagat cgtcttcacg accaagaaca tcctcaacaa ggtcctcgag gtgcagtcgc
     1021 gcaccaactt catcaagcgc atcatcatcc tggacaccgt ggaaaacatc catggctgcg
     1081 agtccctgcc caacttcatc tcgcggtact cggacggtaa catcgccaac ttcaagcccc
     1141 tccacttcga ccccgtcgag caggtcgcgg ccatcctgtg ttcgtcgggc accacgggtc
     1201 tgccgaaggg tgtcatgcag acgcatcaga acatctgtgt ccggctgatc cacgccctgg
     1261 acccgcggta cggtacgcag ctgatccccg gcgtgaccgt gctggtgtac ctgcccttct
     1321 tccacgcctt cggtttccat atcaccctcg gttacttcat ggtcggcctg cgggtgatca
     1381 tgttccgccg cttcgaccag gaggccttcc tgaaggcgat ccaggactac gaggtccgca
     1441 gcgtgatcaa cgtcccgtcg gtcatcctgt tcctgagcaa gagcccgctg gtggacaagt
     1501 acgacctctc ctcgctccgc gagctgtgct gcggcgccgc cccgctggcc aaggaagtgg
     1561 cggaggtggc cgcgaagcgc ctgaacctgc cgggtatccg ctgcggcttc ggcctgacgg
     1621 agagcacctc cgcgatcatc cagaccctgg gcgacgagtt caagtcgggt tcgctgggcc
     1681 gggtcacccc cctcatggcg gcgaagatcg cggaccgcga aacgggtaag gccctcggcc
     1741 ccaaccaagt cggtgagctg tgcatcaagg gccccatggt gagcaagggt tacgtgaaca
     1801 acgtcgaggc caccaaggag gcgatcgacg acgacggctg gctgcattcg ggcgacttcg
     1861 gctactacga cgaagacgag catttctacg tggtggaccg gtacaaggaa ctgatcaagt
     1921 acaagggcag ccaggtcgcg cccgcggagc tggaggaaat cctgctgaag aacccgtgta
     1981 tccgcgacgt cgccgtcgtc ggcatccccg acctggaggc gggtgaactg ccgtcggcct
     2041 tcgtcgtgaa gcagcccggc acggagatca ccgccaagga ggtctacgac tacctggccg
     2101 agcgcgtgtc ccacaccaag tacctgcgcg gcggcgtccg gttcgtggac tcgatcccgc
     2161 gcaacgtgac cggcaagatc acccggaagg aactcctgaa gcaactcctc gtgaaggccg
     2221 gcggctaatt aattaagcat gcggccggcc agtactctgc agctggatcc atggatatct
     2281 agatttaaag atctggtacc gcggccgcgc cacggatgcc accacaggca ctacaacgga
     2341 gttcgccacg tacatcacca caaccaccga ttctggcggt gagctccccg atattcagcg
     2401 gaaatggctt ggtatcgacc aagattcgta gaaccccgtc tcgtctggct ggtattcaaa
     2461 acgggcgcaa cgaaacacgc aacgagacag gcatggccca aaccagaaaa ctagcgtcta
     2521 ccaggacttt tacctgtccg acccgttgca acggaacccc ccacggaacc cccgcgacac
     2581 ccgctcccca attgcgttag aacagcggtg gattgtcggc ttcgttgtgg gccttttgag
     2641 ccgcttcctg ttctgccgca cgctctttcc tcgcccgata gccgagtcgc ttaacggtgt
     2701 ccagatgcag cccgaaatgt ttggccgttt gcggccaaga gtggccctcg tcgtcgtgat
     2761 aggcgcggat gcgttcgcgg cgtgcagcct gctcggcgag ccactcgctg cgttcctgcg
     2821 ccacgagccg gacgacgtgg cgttcggata gtccggtgat tcgagcgcct tcggcggcgg
     2881 tcacgcgccg ctttttgcgg acagtcggct gccggttgta gccgtcgctg tagccgtcgc
     2941 tgtagccgtc gctcatagca atgcctccat gcatggctga cgcggacttt gcgcgccgcg
     3001 caactgtgct cgccgccgtg cgcgctgctg cgcccttccg cgagatggcc gactggcgcg
     3061 cactgagtgt ggcctcgtag accacgatcc cgtccgccca aatgcgcgac ttggttgtga
     3121 tccaacgcca aatgctgttg gcgatggcgc ggacctcgct gtccggtagc ggtccgggac
     3181 acacgtcgtt gcacggaaat tcggcgtttc gcgcgtggca ctcggcatag atcgcgcggc
     3241 cgagtccgtc cacgttccgg gtcggcaggt agatccgcat gagggcggga cgataggccc
     3301 acaacctgac ggaatcgaac agtgcgcaat tccgccctag cggcgtcgga gccgctttgt
     3361 acgtggtctg ctgacgccag cgcggcggtg gcatgttcgc gccgagctcg gcctcgatgt
     3421 ggctgagtgt gtagagatct gagtggagcc attccgtttc ccaggcgatg tggccggggt
     3481 ttttggtcat gaggcctgag taactgcggt cgccatcgac ggcgcgccga aggccttcgg
     3541 cgcacgccgc catgtatgcg agcggcttac gccgcgcgta ttcggtgcgt ggaacagggg
     3601 cgttgagtgc ccacactgcg tgtgcgtggc cgttggcgcg attgcccacg atcgcgttgg
     3661 gcagcggatg ggacccccgg gcgctgagcg ctcggagcgc tgcgtctgga tggtctacgt
     3721 ccacgaccag caggtttgcc agcgctgttg ggttcgcctc gatgtaccgg cggcctaggg
     3781 ccgacgcgcg gctttggcgg tagatcccct cgagcagatc gtcgcttgcc agcggccagt
     3841 acggcagcca gagctgctca aattcgtcgg cgacgtggct cacgcttggt agtagaccac
     3901 gattaatcac cggtgtatgg tccgacacga gctccaagtc agatatttcg ctgaggggcc
     3961 accccacaac tgcacactcc cccgctctcc cgtcgagccc tggtggtgga acaccagcga
     4021 cagccgagca cccccaacca cctgtaccaa ccaggaggaa cacatgcgtc gtttcgagga
     4081 cgtttccggg ccgctgagag ccgctgtggc ggccgtacac gccgccttag acccgttaga
     4141 ccccctgccg cctgaatgcg cgggtacgag ccacacagcg cccgaactta cggagctggt
     4201 gggctcacgc gtgcggccgc acgcgttgcg ctcggtcgtt cggctgcggc gagcggtatc
     4261 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4321 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4381 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4441 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4501 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4561 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4621 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4681 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4741 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4801 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     4861 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4921 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     4981 gatcttttct acggggtctg acgctcagtg gaactagagc atgagattat caaaaaggat
     5041 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     5101 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     5161 tctatttcgt tcatccatag ttgcctgact ccccgacgtg gccgaccagc ccgtcatcgt
     5221 caacgcctga ccgcggtgcg gacaggccgt gtcgcgaccg gccgtgcgga attaagccgg
     5281 cccgtaccct gtgaatagag gtccgctgtg acacaagaat ccctgttact tctcgaccgt
     5341 attgattcgg atgattccta cgcgagcctg cggaacgacc aggaattctg ggagccgctg
     5401 gcccgccgag ccctggagga gctcgggctg ccggtgccgc cggtgctgcg ggtgcccggc
     5461 gagagcacca accccgtact ggtcggcgag cccgacccgg tgatcaagct gttcggcgag
     5521 cactggtgcg gtccggagag cctcgcgtcg gagtcggagg cgtacgcggt cctggcggac
     5581 gccccggtgc cggtgccccg cctcctcggc cgcggcgagc tgcggcccgg caccggagcc
     5641 tggccgtggc cctacctggt gatgagccgg atgaccggca ccacctggcg gtccgcgatg
     5701 gacggcacga ccgaccggaa cgcgctgctc gccctggccc gcgaactcgg ccgggtgctc
     5761 ggccggctgc acagggtgcc gctgaccggg aacaccgtgc tcacccccca ttccgaggtc
     5821 ttcccgaact gctgcgggac gccgcgcggc gccgctgagg accaccgcgg gtggggctac
     5881 ctctggcccc ggctgctgga ccgcctggag gactggctgc cggacgtgga cacgctgctg
     5941 gccggccgcg aaccccggtt cgtccacggc gacctgcacg ggaccaacat cttcgtggac
     6001 ctggccgcga ccgaggtcac cgggatcgtc gacttcaccg acgtctatgc gggagactcc
     6061 cgctacagcc tggtgcaact gcatctcaac gccttccggg gcgaccgcga gatcctggcc
     6121 gcgctgctcg acggggcgca gtggaagcgg accgaggact tcgcccgcga actgctcgcc
     6181 ttcaccttcc tgcacgactt cgaggtgttc gaggagaccc cgctggatct ctccggcttc
     6241 accgatccgg aggaactggc gcagttcctc tgggggccgc cggacaccgc ccccggcgcc
     6301 tgacgccccc tctagctgat caccgcggcc atgatggcat aaaacgaaag gcccagtctt
     6361 tcgactgagc ctttcgtttt atttgatgcc tggcagtcga tcgtacgcta gttaactacg
     6421 tcgacatcga taagcttggt accgagctcg gatccactag
//
