LOCUS       sequence-101578-         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson JH
  TITLE     Drosophila Brainbow: a recombinase-based fluorescence labeling 
            technique to subdivide neural expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):253-9. doi: 10.1038/nmeth.1566. Epub 2011
            Feb 6.
  PUBMED    21297621
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1566"; journalName: "Nat Methods"; date: "2011-03"; 
            volume: "8"; issue: "3"; pages: "253-9"
COMMENT     Sequence Label: dBrainbow
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             240..266
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             270..296
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             300..326
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    352..385
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tgcgcgacgg cgtgctgaag ggcgacgtca agcacaagct gctgctggag ggcggcggcc
       61 accaccgcgt tgacttcaag accatctaca gggccaagaa ggcggtgaag ctgcccgact
      121 atcactttgt ggaccaccgc atcgagatcc tgaaccacga caaggactac aacaaggtga
      181 ccgtttacga gagcgccgtg gcccgcaact ccaccgacgg catggacgag ctgtacaagt
      241 acccatacga tgttcctgac tatgcgggct atccctatga cgtcccggac tatgcaggat
      301 atccatatga cgttccagat tacgctggcg gataacgtaa gctagggtac cataacttcg
      361 tataatgtat gctatacgaa gttatcaaaa tgataccggt cggagtaagc aaaggtgagg
      421 agagcgtgat caaacccgag atgaagatgc gctactatat ggacggcagc gtcaacggcc
      481 acgagtttac catcgagggc gagggcacgg gacgcccgta cgaaggacac caggagatga
      541 ccctgcgcgt gaccatggcc gagggtggcc cgatgccctt cgccttcgat ctggtgagcc
      601 acgtgttctg ctacggtcac cgcgtcttca ccaaataccc cgaggaaatc cccgattact
      661 tcaagcaagc cttcccggag ggcctgtcgt gggagcgcag cctggagttc gaggatggcg
      721 gaagcgccag cgtgagcgcg cacatcagcc tgcggggcaa caccttctac cataagagca
      781 agttcaccgg cgtgaatttc ccagccgatg gccccatcat gcagaaccag agtgtggatt
      841 ggg
//
