LOCUS       sequence-101582-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To study the effect of DPYS activity on EMT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Shaul YD, Freinkman E, Comb WC, Cantor JR, Tam WL, Thiru P, Kim D, 
            Kanarek N, Pacold ME, Chen WW, Bierie B, Possemato R, Reinhardt F, 
            Weinberg RA, Yaffe MB, Sabatini DM
  TITLE     Dihydropyrimidine accumulation is required for the 
            epithelial-mesenchymal transition.
  JOURNAL   Cell. 2014 Aug 28;158(5):1094-109. doi: 10.1016/j.cell.2014.07.032.
  PUBMED    25171410
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.07"; journalName: "Cell"; date: "2014-08-28- 
            28"; volume: "158"; issue: "5"; pages: "1094-109"
COMMENT     Sequence Label: DPYS-FLAG
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..641
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(42..61)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     538..558
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 tcaccgacct ctctccccag ggggatccac cggagcttac catgaccgag tacaagccca
       61 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
      121 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
      181 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
      241 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
      301 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
      361 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
      421 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
      481 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
      541 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
      601 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc
      661 agcgcccgac cgaaaggagc gcacgacccc atgcatcggt acctttaaga ccaatgactt
      721 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
      781 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttaga
//
