LOCUS       sequence-101609-         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  integration of M. smegmatis ald gene in Mycobacterium using 
            mycobacteriophage L5 attB site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Feng Z, Caceres NE, Sarath G, Barletta RG
  TITLE     Mycobacterium smegmatis L-alanine dehydrogenase (Ald) is required 
            for proficient utilization of alanine as a sole nitrogen source and 
            sustained anaerobic growth.
  JOURNAL   J Bacteriol. 2002 Sep;184(18):5001-10.
  PUBMED    12193615
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol."; date: "2002-09"; volume: "184(18)"; pages: "5001-10"
COMMENT     Sequence Label: pBUN279
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     90..108
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     135..155
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        137..155
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(197..216)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(198..216)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aataacaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgnac gtctaagaaa
       61 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
      121 aagaattcgc ggccgcaatt aaccctcact aaagggatct taattaagga tcctgatcag
      181 gcgccttaat taagatccct atagtgagtc gtattatgcg gccgcgaatt ctcatgtttg
      241 accgcttatc atcggatccc cgaggataat tcggcttcgg atcacaccga tctcctgcaa
      301 ccggcgaacg cgtccgtggc acgtcgacgg agctatcccg accatctcgg ccagcgcact
      361 gctgggtatg cgtgcatcgt cgtgcaacgc cagcaaaatg cggcggtcga tgtcgtcgag
      421 gtcgcgggcc cgaacatcct tcggcgaacc gggcgtttgg acgcccgtga tcgatgatcc
      481 ttcactcacg gcacaatcct aacgaatttt cgacacagat attgctcaca tttcgaaggt
      541 tcttcacact tagaggtgac attacccctt ctgaaggagc gatcatgctc gtcggaatcc
      601 cgaccgagat caagaacaac gagtaccggg tcgccatcac cccggctggt gtggtagagc
      661 tgacccgcaa gaggtcacga ggtgatcatc caggccggt
//
