LOCUS       sequence-101612-         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GeneGuard genomic cassette: for constructing Delta-dapA::RBS E pi, 
            Kis, FRT-kanR-FRT (medium-copy support for R6K COR). Will support 
            pSEVAD1K..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Wright O, Delmans M, Stan GB, Ellis T
  TITLE     GeneGuard: a Modular Plasmid System Designed for Biosafety.
  JOURNAL   ACS Synth Biol. 2014 May 13.
  PUBMED    24847673
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 May 13."
COMMENT     Sequence Label: pSEVA12DKR-E
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(40..717)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
ORIGIN
        1 tctttcgact gagcctttcg ttttatttga tgcctttaat taagcaccgg tggagtgacg
       61 accttcagca cgttcgtact gttcaacgat ggtgtagtct tcgttgtggg aggtgatgtc
      121 cagtttgatg tcggttttgt aagcacccgg cagctgaacc ggttttttag ccatgtaggt
      181 ggttttaact tcagcgtcgt agtgaccacc gtctttcagt ttcagacgca ttttgatttc
      241 acctttcaga gcaccgtctt ccgggtacat acgttcggtg gaagcttccc aacccatggt
      301 ttttttctgc ataaccggac cgtcggacgg gaagttggta ccacgcagtt taactttgta
      361 gatgaactca ccgtcttgca gggaggagtc ctgggtaacg gtaacaacac caccgtcttc
      421 gaagttcata acacgttccc atttgaaacc ttccgggaag gacagtttca ggtagtccgg
      481 gatgtcagcc gggtgtttaa cgtaagcttt ggaaccgtac tggaactgcg gggacaggat
      541 gtcccaagcg aacggcagcg gaccaccttt ggtaactttc agtttagcgg tctgggtacc
      601 ttcgtacgga cgaccttcac cttcaccttc gatttcgaac tcgtgaccgt taacggaacc
      661 ttccatacga actttgaaac gcatgaactc tttgataacg tcttcggagg aagccatcta
      721 gtattcacca cctttctcta gtaacattta ttgtacaaca cgagcccatt tttgtcaaat
      781 aaaatttaaa ttatatcaac gttaataaga cgttgtc
//
