LOCUS       sequence-101616-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GeneGuard genomic cassette: for constructing Delta-dapA::RBS E pi, 
            Kis, FRT-kanR-FRT (medium-copy support for R6K COR). Will support 
            pSEVAD1K..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 635 to 669)
  AUTHORS   Wright O, Delmans M, Stan GB, Ellis T
  TITLE     GeneGuard: a Modular Plasmid System Designed for Biosafety.
  JOURNAL   ACS Synth Biol. 2014 May 13.
  PUBMED    24847673
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 May 13."
COMMENT     Sequence Label: pSEVA12DKR-E
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        635..669
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
ORIGIN
        1 atctagtatt caccaccttt ctctagtaac atttattgta caacacgagc ccatttttgt
       61 caaataaaat ttaaattata tcaacgttaa taagacgttg tcaataaaat tattttgaca
      121 aaattcctag gcgtcgaaaa gtgctgtgaa gcgttcaatt aaatgcgggg agtctgccac
      181 atcgacctga acccagacag atgctggcat tggcggacgc ggacgcgccg tcgtgcgctt
      241 catcacgatt aaaagatcca gttcggcacc tttcaacggt aacgttgatt caatcagagt
      301 aatggcattc catgaaccgg aaagcagcat aataaacgtg aactcttctc ccagcatcgc
      361 caggcgactg tcttcaatat tacagccgca actactgaca tgacgggtga tggtgttcac
      421 aattccaggg cgatcggcac ccaacgcagt gatcaccaga taatgttgcg atgacagtgt
      481 caaactggtt attcctttaa ggggtgagtt gttcttaagg aaagcataaa aaaaacatgc
      541 atacaacaat cagaacggtt ctgtctgctt gcttttaatg ccataccaaa cgtaccattg
      601 agacacttgt ttgcacagag gatggcccga attcttgacg gctagctcag tcctaggtac
      661 agtgctagcg gatcttccgg aagactaggt gagactcaag gtcatgatgg acgtgaacaa
      721 aaaaacgaaa attcgccacc gaaacgagct aaatcacacc ctggctcaac ttcctttgcc
      781 cgcaaagcga gtgatgtata tggcgcttgc tcccattgat agcaaagaac ctcttgaacg
      841 agggcgagtt ttcaaaat
//
