LOCUS       sequence-101617-        1197 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expression of C. briggsae gld-1, used for rescue of C. elegans 
            q485 homozygotes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Beadell AV, Liu Q, Johnson DM, Haag ES
  TITLE     Independent recruitments of a translational regulator in the 
            evolution of self-fertile nematodes.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Dec 6;108(49):19672-7. doi: 
            10.1073/pnas.1108068108. Epub 2011 Nov 21.
  PUBMED    22106259
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1108068108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-12-6- 6"; volume: "108"; issue: "49"; pages: "19672-7"
COMMENT     Sequence Label: pAD-g6
FEATURES             Location/Qualifiers
     source          1..1197
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    586..610
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(672..692)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gggcccaaag caaagcagga aattaatttc ataaactttc acttgattga aaaattccaa
       61 aaatactccn aaactttttc acgatgagca tgatttgacg tcatgagagg cccagacgtg
      121 cgaagaaata aaaattgcgg tcataaactg aaacgtaaca tatgataagg tattttgtgt
      181 gcggtttttt ctatgatgtt tcaaaaaaat ttatcagaag taaaaaacat aagaagtgta
      241 cacagaacat tgtgtagatt ttgggataga attattatta atggggggag ggagcacaat
      301 ttttttcgac tttttattac gaattaattc tatttttgaa tctacacaat ttcattgtta
      361 gaggtgactt aaaagaagct aaaaaacaaa gaaattaaga gaagtttttt gataataaca
      421 aaaatagggg gtgggagcac agggagaaag agcatgtagg gatgttgaag agtaattgga
      481 cttagaagtc agaggcacgg gcgcgagatg ctgaaaattt aaatattact cgttagttag
      541 tagaactcag tttaaactta ctctgatgac agcggcccct attatcccac tttgtacaag
      601 aaagctgggt cggcgcgccc acccttgggc ttttgtatag ttcgtccatg ccatgtgtaa
      661 tcccagcagc tgttacaaac tcaagaagga ccatgtggtc tctcttttcg ttgggatctt
      721 tcgaaagggc agattgtgtg gacaggtaat ggttgtctgg taaaaggaca gggccatcgc
      781 caattggagt attttgttga taatggtctg ctagttgaac gcttccatct tcaatgttgt
      841 gtctaatttt gaagtttctg aaaatttnaa tcagattagt tagttagtaa aatcatggtt
      901 taaacttaca actttgattc cattcttttg tttgtctgcc atggatgtat acnttgtgtg
      961 agttatagtt ggtatttccn atttgtgtcc cagaatgttt tccntcttct ttaaaaacca
     1021 ataccctttt aactcnnatt cnaattaacc agggtatccc ccttccaacc tggacttccg
     1081 cccccggaaa attnaaaatg gtatggttag ttagtacccn accggtttaa acttaccggn
     1141 nctggantnc cgcccccttt gnaaaaaaag gtctttccgg acataccctt cgggnng
//
