LOCUS       sequence-101619-         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expression of C. briggsae gld-1, used for rescue of C. elegans 
            q485 homozygotes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Beadell AV, Liu Q, Johnson DM, Haag ES
  TITLE     Independent recruitments of a translational regulator in the 
            evolution of self-fertile nematodes.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Dec 6;108(49):19672-7. doi: 
            10.1073/pnas.1108068108. Epub 2011 Nov 21.
  PUBMED    22106259
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1108068108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-12-6- 6"; volume: "108"; issue: "49"; pages: "19672-7"
COMMENT     Sequence Label: pAD-g6
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             288..311
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             312..338
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(342..366)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttttccagag cccgacaatc gctggatcaa gtggaattct cggaaacaac atcttcgact
       61 actccctgtt gacaccgagc atgttcgact cgtttagctc cctccaactg gctagtgacc
      121 tgacgttccc gaactatcca acaaccactt cgtttgtcaa ttcgttcccg ggactcttca
      181 cctcttcttc atcgaacgtg acaccatcag tcaacagtac aagcaccact caagctcaat
      241 caggaggaga cagtccatcg gcttcttcgg tcaacaacac ctctttcgac tacaaggacg
      301 acgacgacaa gtacccatac gacgtcccag actacgctta gacccagctt tcttgtacaa
      361 agtggaacat agcataattc tcacaccaac attcttcgta gtttttgttc tgttagttct
      421 tactactttc aaatatacac agtgctagca tagaatcatg taccatatat cgtgtatcca
      481 tcaccccctc caccctgtcc ctttcccttg tttttttctt cccttttttt tggtatctgc
      541 catctcacat cctttaggaa tagcatatac atacaaatgt aacaattatg tcatgagatc
      601 ccttaatacc attaattctt ttctgtaatt taaactcgcc ccccaatata tgcaatcatt
      661 cccagtatta tccttgtttt cctgttgcag gacatcaaag tttaatatag gttgaagtct
      721 acacaatctc gactttcccc cgtaatggcc ctct
//
