LOCUS       sequence-101627-        1213 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express pHluorin-mKate2-hLC3 (PK-hLC3) in mammalian cells for 
            monitoring autophagy, based on FUGW (3rd gen lentiviral plasmid).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Tanida I, Ueno T, Uchiyama Y
  TITLE     A Super-Ecliptic, pHluorin-mKate2, Tandem Fluorescent Protein-Tagged
            Human LC3 for the Monitoring of Mammalian Autophagy.
  JOURNAL   PLoS One. 2014 Oct 23;9(10):e110600. doi: 
            10.1371/journal.pone.0110600. eCollection 2014.
  PUBMED    25340751
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-10-23"; volume: "9(10):e110600. doi"; pages: " 
            10.1371/journal.pone.0110600. eCollection 2014"
COMMENT     Sequence Label: FUGW-PK-hLC3
FEATURES             Location/Qualifiers
     source          1..1213
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(223..251)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tnnnnnnnng nggnggggnc gnggtgtttg ggnagttttt taggcacctt ttgaaatgat
       61 aatcatttgg gtcaatatgt aattttcagt gttagactag taaattgtcc gctaaattct
      121 ggccgttttt ggcttttttg ttagacgaag cttgggctgc aggtcgactc tagaggatcc
      181 gctagcgcca ccatgagtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
      241 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
      301 gcaacatacg gaaaacttac ccttaaattt atttgcacna ctggaaaact acctgttcca
      361 tggccaacac ttgtcacnac gttaacttat ggtgttcaat gcttttcaag atacccagat
      421 cagatgaaac gncatgactt tttcaagaga gccatgcccg aangttacgt acaggaaana
      481 acnataatnt ncaaaganga cggcaaacna caacgacccn cgcagaancc cnagtatnga
      541 nnngtgatcc cccncngatc nnncaannga tnaaacntgt ancgnacgta cacaatactg
      601 gaaaccncnc tcngannccg gnanntcgnn naccacnctg aaacnccacg cacaanctgt
      661 ccgaccnntn ncgannccac acanannnct cacnnnannn gnnnncncaa nnnncgnnnn
      721 ncactaancc agannnngca cgccgcnggc catgncnnaa anccnncgag nacgnntcaa
      781 tgancanccn agcccnngaa gnnnttaanc nactcnagcc anannccaan nacntangnn
      841 annaagnann ngcaancnna ccncacccca atcaancann cccacnntcn ncggaccnan
      901 cccccnancc nagcncttaa gnnacanncn nnanccnnaa actgatcctc canngaanca
      961 cncaaanacg nccccccnan cnanacngac nnaaacnaag gannncnacc ctaaaaganc
     1021 gagaccnanc tnnncacnna nnnnatncan anacnancga acnangcaaa nnaccccaga
     1081 cnnaaagncn nnnnggcnat ctncccaccn atncctaccc acnaancnnt ccctcgcccg
     1141 taanccantn cccaaanacn ncgantnnta gnnnncnctc nntccncnnc ctnngncnca
     1201 naaatnnnan cgc
//
