LOCUS       sequence-101628-         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express pHluorin-mKate2-hLC3 (PK-hLC3) in mammalian cells for 
            monitoring autophagy, based on FUGW (3rd gen lentiviral plasmid).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Tanida I, Ueno T, Uchiyama Y
  TITLE     A Super-Ecliptic, pHluorin-mKate2, Tandem Fluorescent Protein-Tagged
            Human LC3 for the Monitoring of Mammalian Autophagy.
  JOURNAL   PLoS One. 2014 Oct 23;9(10):e110600. doi: 
            10.1371/journal.pone.0110600. eCollection 2014.
  PUBMED    25340751
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-10-23"; volume: "9(10):e110600. doi"; pages: " 
            10.1371/journal.pone.0110600. eCollection 2014"
COMMENT     Sequence Label: FUGW-PK-hLC3
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             295..669
                     /codon_start=1
                     /gene="MAP1LC3B"
                     /product="human LC3 protein"
                     /label=LC3B
                     /note="Atg8 family member found on autophagosomes"
                     /translation="MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLP
                     VLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKD
                     EDGFLYMVYASQETFGMKLSV"
ORIGIN
        1 tccaccgaga ccctgtaccc cgctgacggc ggcctggaag gcagagccga catggccctg
       61 aagctcgtgg gcgggggcca cctgatctgc aacttgaaga ccacatacag atccaagaaa
      121 cccgctaaga acctcaagat gcccggcgtc tactatgtgg acagaagact ggaaagaatc
      181 aaggaggccg acaaagagac ctacgtcgag cagcacgagg tggctgtggc cagatactgc
      241 gacctcccta gcaaactggg gcacagaggt ggaggaggtt ccggactcag atctatgccg
      301 tcggagaaga ccttcaagca gcgccgcacc ttcgaacaaa gagtagaaga tgtccgactt
      361 attcgagagc agcatccaac caaaatcccg gtgataatag aacgatacaa gggtgagaag
      421 cagcttcctg ttctggataa aacaaagttc cttgtacctg accatgtcaa catgagtgag
      481 ctcatcaaga taattagaag gcgcttacag ctcaatgcta atcaggcctt cttcctgttg
      541 gtgaacggac acagcatggt cagcgtctcc acaccaatct cagaggtgta tgagagtgag
      601 aaagatgaag atggattcct gtacatggtc tacgcctccc aggagacgtt cgggatgaaa
      661 ttgtcagtgt aagaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt
      721 tgtgaaagat tgact
//
