LOCUS       sequence-101630-         524 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express pHluorin-mKate2-hLC3∆G (PK-hLC3∆G), a negative control for 
            pHluorin-mKate2-hLC3 (PK-hLC3), in mammalian cells for monitoring 
            autophagy, based on FUGW (3rd gen lentiviral plasmid).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Tanida I, Ueno T, Uchiyama Y
  TITLE     A Super-Ecliptic, pHluorin-mKate2, Tandem Fluorescent Protein-Tagged
            Human LC3 for the Monitoring of Mammalian Autophagy.
  JOURNAL   PLoS One. 2014 Oct 23;9(10):e110600. doi: 
            10.1371/journal.pone.0110600. eCollection 2014.
  PUBMED    25340751
REFERENCE   2  (bases 1 to 524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-10-23"; volume: "9(10):e110600. doi"; pages: " 
            10.1371/journal.pone.0110600. eCollection 2014"
COMMENT     Sequence Label: FUGW-PK-hLC3∆G
FEATURES             Location/Qualifiers
     source          1..524
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             80..451
                     /codon_start=1
                     /gene="MAP1LC3B"
                     /product="human LC3 protein"
                     /label=LC3B
                     /note="Atg8 family member found on autophagosomes"
                     /translation="MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLP
                     VLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKD
                     EDGFLYMVYASQETFMKLSV"
ORIGIN
        1 cgaggtggct gtggccagat actgcgacct ccctagcaaa ctggggcaca gaggtggagg
       61 aggttccgga ctcagatcta tgccgtcgga gaagaccttc aagcagcgcc gcaccttcga
      121 acaaagagta gaagatgtcc gacttattcg agagcagcat ccaaccaaaa tcccggtgat
      181 aatagaacga tacaagggtg agaagcagct tcctgttctg gataaaacaa agttccttgt
      241 acctgaccat gtcaacatga gtgagctcat caagataatt agaaggcgct tacagctcaa
      301 tgctaatcag gccttcttcc tgttggtgaa cggacacagc atggtcagcg tctccacacc
      361 aatctcagag gtgtatgaga gtgagaaaga tgaagatgga ttcctgtaca tggtctacgc
      421 ctcccaggag acgttcatga aattgtcagt gtaagaattc gatatcaagc ttatcgataa
      481 tcaacctctg gattacaaaa tttgtgaaag attgactggt attc
//
