LOCUS       sequence-101631-         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Chang MC, Eachus RA, Trieu W, Ro DK, Keasling JD
  TITLE     Engineering Escherichia coli for production of functionalized 
            terpenoids using plant P450s.
  JOURNAL   Nat Chem Biol. 2007 May . 3(5):274-7.
  PUBMED    17438551
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2007 May . 3(5):274-7."
COMMENT     Sequence Label: pCWori-A13AMO-aaCPRct
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(96..101,102..119,120..126)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    134..150
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(205..210,211..226,227..233)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    241..257
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(300..305,306..321,322..328)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    336..352
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 atggtgtcct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
       61 aatgtaagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
      121 ataatgtgtg gaattgtgag cggataacaa tttcacacag gaaacaggat cgatccatcg
      181 atgagcttac tccccatccc cctgttgaca attaatcatc ggctcgtata atgtgtggaa
      241 ttgtgagcgg ataacaattt cacacaggaa acaggatcag cttactcccc atccccctgt
      301 tgacaattaa tcatcggctc gtataatgtg tggaattgtg agcggataac aatttcacac
      361 aggaaacagg atccatcgat gcttaggagg tcatatgacc gtacacgaca tcatcgcaac
      421 gtacttcact aaatggtacg taattgtgcc gctggcactg attgcgtatc gcgtgctgga
      481 ttatttctac gcgacgcgct ccaaaagcac caagaaatct ctgccagaac cgtggcgcct
      541 gccgatcatt ggtcacatgc accacctgat tggtacgacc ccgcatcgcg gtgttcgtga
      601 cctggcccgt aaatacggct ccctgatgca cctgcagctg ggtgaagtgc cgacgatcgt
      661 tgtctcttcc ccgaaatggg cgaaagagat cctgactacc tacgatatca ccttcgccaa
      721 ccgtccggaa actctgaccg gcgaaatcgt actgtaccat aacactgatg ttgtgctggc
      781 gccgtatggg tgagtactgg cgtcagct
//
