LOCUS       sequence-101638-         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Low core fragment expression (low resource allocator).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Segall-Shapiro TH, Meyer AJ, Ellington AD, Sontag ED, Voigt CA
  TITLE     A 'resource allocator' for transcription based on a highly 
            fragmented T7 RNA polymerase.
  JOURNAL   Mol Syst Biol. 2014 Jul 30;10(7):742.
  PUBMED    25080493
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-07-30"; volume: "10(7)"; pages: "742"
COMMENT     Sequence Label: pTHSSd_39
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..24)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      complement(110..141)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 aactcaatga tgatgatgat gatggtcgac ggcgctattc agatcctctt ctgagatgag
       61 ttattaccgc ctttgagtga gccccaatga taaccccaag ggaagtttta gtcaaaagcc
      121 tccggtcgga ggcttttgac tttctgctaa tctaactaac taacccttag tgactcctgc
      181 agcggccgca ctagtattat ttgtaagctt cgatttcttt acgcaggttc gcgattttct
      241 gcttcagttg ttcacgtttc tgtttcagct gttcgatacg gttgcgcagt tccgcttttt
      301 tggatttcag ttcttctttt tcgttggatc caccacctga acctccgttc tcatcggtca
      361 cggtaactac ttcgttatcg gtcccattga ttgcgtctgc ttgtagaatc tcgttgactt
      421 tcttagcaac aatcccgtag atgtcctgaa cggtttcact aggaagcaag ttaaccgcgc
      481 gaccacctac ctcatctcgg agcatcgcgg agaagtgctg gatgccagag caagacccgt
      541 caaacgccag cggaagggag cagttatagc tcaggccgtg gtgctgtacc ccagcgtact
      601 caaagcagaa cgcaaggaag cagaacggag aatcttgctc agcccaccaa gtgttctcca
      661 gtggagactt agcgcaagcc atgatgttct cgtggttttc ctcaatgaac ttgatgcgct
      721 cagggaacgg aaccttgtcg acacccgcac agtttgcacc gtggatt
//
