LOCUS       sequence-101644-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  neomycin resistance; constitutive promoter driving reverse tet 
            activator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Sim X, Cardenas-Diaz FL, French DL, Gadue P
  TITLE     A Doxycycline-Inducible System for Genetic Correction of iPSC 
            Disease Models.
  JOURNAL   Methods Mol Biol. 2015 Jan 29.
  PUBMED    25630922
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol. 2015 Jan 29."
COMMENT     Sequence Label: AAVS1-SA-2A-NEO-CAG-RTTA3
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..705
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="SRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHVK
                     NKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTRP
                     TEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTTD
                     SMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPAD
                     ALDDFDLDMLPG"
ORIGIN
        1 tctaggctgg acaagagcaa agtcataaac ggagctctgg aattactcaa tggtgtcggt
       61 atcgaaggcc tgacgacaag gaaactcgct caaaagctgg gagttgagca gcctaccctg
      121 tactggcacg tgaagaacaa gcgggccctg ctcgatgccc tgccaatcga gatgctggac
      181 aggcatcata cccacttctg ccccctggaa ggcgagtcat ggcaagactt tctgcggaac
      241 aacgccaagt cataccgctg tgctctcctc tcacatcgcg acggggctaa agtgcatctc
      301 ggcacccgcc caacagagaa acagtacgaa accctggaaa atcagctcgc gttcctgtgt
      361 cagcaaggct tctccctgga gaacgcactg tacgctctgt ccgccgtggg ccactttaca
      421 ctgggctgcg tattggagga acaggagcat caagtagcaa aagaggaaag agagacacct
      481 accaccgatt ctatgccccc acttctgaga caagcaattg agctgttcga ccggcaggga
      541 gccgaacctg ccttcctttt cggcctggaa ctaatcatat gtggcctgga gaaacagcta
      601 aagtgcgaaa gcggcgggcc gaccgacgcc cttgacgatt ttgacttaga catgctccca
      661 gccgatgccc ttgacgattt tgaccttgac atgctccccg ggtaaacgcg tgaattcact
      721 cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc cctggctcac
      781 aaataccact gagatctttt
//
