LOCUS       sequence-101647-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Sec61-N-18
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..309)
                     /codon_start=1
                     /gene="SEC61B"
                     /product="human Sec61-beta subunit of the translocon in the
                     endoplasmic reticulum"
                     /label=Sec61-beta
                     /translation="MPGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAG
                     RTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS"
     promoter        complement(344..547)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(373..393)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        548..851
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cgtcgactgc agaattcgtc ccgaacgagt gtacttgccc caaatgtgca acataaatac
       61 agaagcgatg aacagaagac tcataaccaa tactggaaca gggccaactt tgagcccagg
      121 tgaatcttct gtgtagaatc gccacatccc cccggtgcct gccgaggttg tgcggcctgc
      181 actccttgtc ccacagctgg catttttcct ctgccggaca gtggatcccg ccgcccgggc
      241 ggccactgct ttgctgggag agcgccctga ggatcccacg ttagtgccac tgggggtcgg
      301 accaggcatg gtggcgctag cggatctgac ggttcactaa accagctctg cttatataga
      361 cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt
      421 ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga
      481 gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc
      541 gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc
      601 ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag
      661 ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat
      721 actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca
      781 ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa
      841 gttatgtaac gcggaactcc atatatgg
//
