LOCUS       sequence-101649-         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Sec61-C-18
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             295..582
                     /codon_start=1
                     /gene="SEC61B"
                     /product="human Sec61-beta subunit of the translocon in the
                     endoplasmic reticulum"
                     /label=Sec61-beta
                     /translation="PGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAGR
                     TTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS"
     polyA_signal    743..864
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(780..799)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     834..853
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 cccgaggacg gcgccctgaa gagcgagatc aagaagaggc tgaagctgaa ggacggcggc
       61 cactacgccg ccgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
      121 gcctacatcg tcgacatcaa gttggacatc gtgtcccaca acgaggacta caccatcgtg
      181 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
      241 tccggactca gatctggctc cagcgcaggc agcgcatccg gcggaagcgg aagccctggt
      301 ccgaccccca gtggcactaa cgtgggatcc tcagggcgct ctcccagcaa agcagtggcc
      361 gcccgggcgg cgggatccac tgtccggcag aggaaaaatg ccagctgtgg gacaaggagt
      421 gcaggccgca caacctcggc aggcaccggg gggatgtggc gattctacac agaagattca
      481 cctgggctca aagttggccc tgttccagta ttggttatga gtcttctgtt catcgcttct
      541 gtatttatgt tgcacatttg gggcaagtac actcgttcgt aggaattctg cagtcgacgg
      601 taccgcgggc ccgggatcca ccggatctag ataactgatc ataatcagcc ataccacatt
      661 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      721 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      781 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      841 gtccaaactc atcaatgtat cttaacgcgt a
//
