LOCUS       sequence-101657-         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 383, Emission: 448.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  TITLE     Davidson Blue Fluorescent Proteins
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EBFP2-Beta-Catenin-20
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             146..181
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 cgccgccggg atcactctcg gcatggacga gctgtacaag tccggactca gatctcgagc
       61 tcaagcttcg aattctgcag tcgacggtac cgcggccacc atggctactc aagctgacct
      121 gatggagttg gacatggcca tggagccgga cagaaaagct gctgtcagcc actggcagca
      181 gcagtcttac ttggattctg gaatccattc tggtgccacc accacagctc cttccctgag
      241 tggcaagggc aaccctgagg aagaagatgt tgacacctcc caagtccttt atgaatggga
      301 gcaaggcttt tcccagtcct tcacgcaaga gcaagtagct gatattgacg ggcagtatgc
      361 aatgactagg gctcagaggg tccgagctgc catgttccct gagacgctag atgagggcat
      421 gcagatccca tccacgcagt ttgacgctgc tcatcccact aatgtccagc gcttggctga
      481 accatcacag atgttgaaac atgcagttgt caatttgatt aactatcagg atgacgcgga
      541 acttgccaca cgtgcaattc ctgagctgac aaaactgcta aacgatgagg accaggtggt
      601 agttaataaa gctgctgtta tggtccatca gctttccaaa aaggaagctt ccagacatgc
      661 catcatgcgc tcccctcaga tggtgtctgc cattgtacgc accatgcaga atacaaatga
      721 tgtagagaca gctcgttgta ctgctgggac tctgcacaac ctttctcacc accgcgaggg
      781 cttgctggcc atctttaagt ctggtggcat cccagcgctg gtgaaaatgc ttgggtcacc
      841 agtggattct gtactgttct acgccatcac gacactgcat aatctcctgc tccatcagga
      901 aggagctaa
//
