LOCUS       sequence-10166-e       11831 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11831)
  AUTHORS   Desgranges ZP, Ahn J, Lazebnik MB, Ashworth T, Lee C, Pestell RC, 
            Rosenberg N, Prives C, Roy AL
  TITLE     Inhibition of TFII-I-dependent cell cycle regulation by p53.
  JOURNAL   Mol Cell Biol. 2005 Dec . 25(24):10940-52.
  PUBMED    16314517
REFERENCE   2  (bases 1 to 11831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2005 Dec . 25(24):10940-52."
COMMENT     Sequence Label: pSFG-TFII-I-GFP delta
FEATURES             Location/Qualifiers
     source          1..11831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             397..988
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    1051..1408
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     primer_bind     1819..1841
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1857..1873
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     2211..2227
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    join(2286..2304,2305..2327,2328..2346,2347..2369,2370..2388,
                     2389..2411,2412..2430,2431..2453,2454..2472,2473..2495,
                     2496..2514,2515..2537,2538..2556)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2589..2627
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2624..2648
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2726..2743
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2753..2770
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     regulatory      5678..5687
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5684..5686,5687..5689,5690..6403)
                     /codon_start=1
                     /product="enhanced blue variant of GFP"
                     /label=EBFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5729..5750)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5990..6009)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6337..6358
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(6419..6992,6993..6995,6996..7004)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6586..6603)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6813..6832
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(7005..7625,7626..8012)
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTI
                     EGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHA
                     DALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(7078..7097)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(9202..9219)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9202..9218)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9211..9233)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9427..9446)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9546..9568
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9607..9625)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9693..9797
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9798..9866,9867..10658)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10016..10035)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10829..11417
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11318..11337
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11571..11588
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11705..11726
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11741..11746,11747..11764,11765..11771)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11779..11795
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11784..11806
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11803..11819
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11803..11819
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagctttgct cttaggagtt tcctaataca tcccaaactc aaatatataa agcatttgac
       61 ttgttctatg ccctaggggg cggggggaag ctaagccagc tttttttaac atttaaaatg
      121 ttaattccat tttaaatgca cagatgtttt tatttcataa gggtttcaat gtgcatgaat
      181 gctgcaatat tcctgttacc aaagctagta taaataaaaa tagataaacg tggaaattac
      241 ttagagtttc tgtcattaac gtttccttcc tcagttgaca acataaatgc gctgctgagc
      301 aagccagttt gcatctgtca ggatcaattt cccattatgc cagtcatatt aattactagt
      361 caattagttg atttttattt ttgacatata catgtgaatg aaagacccca cctgtaggtt
      421 tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat
      481 agagaagttc agatcaaggt caggaacaga tggaacagct gaatatgggc caaacaggat
      541 atctgtggta agcagttcct gccccgctca gggccaagaa cagatggaac agctgaatat
      601 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg ctcagggcca agaacagatg
      661 gtccccagat gcggtccagc cctcagcagt ttctagagaa ccatcagatg tttccagggt
      721 gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc
      781 gcttctgttc gcgcgcttat gctccccgag ctcaataaaa gagcccacaa cccctcactc
      841 ggggcgccag tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc
      901 ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt
      961 gactacccgt cagcgggggt ctttcatttg ggggctcgtc cgggatcggg agacccctgc
     1021 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
     1081 cgattgtcta gtgtctatga ctgattttat gcgcctgcgt cggtactagt tagctaacta
     1141 gctctgtatc tggcggaccc gtggtggaac tgacgagttc ggaacacccg gccgcaaccc
     1201 tgggagacgt cccagggact tcgggggccg tttttgtggc ccgacctgag tcctaaaatc
     1261 ccgatcgttt aggactcttt ggtgcacccc ccttagagga gggatatgtg gttctggtag
     1321 gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg gtttgggacc
     1381 gaagccgcgc cgcgcgtctt gtctgctgca gcatcgttct gtgttgtctc tgtctgactg
     1441 tgtttctgta tttgtctgaa aatatgggcc cgggctagcc tgttaccact cccttaagtt
     1501 tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca
     1561 agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc
     1621 cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac
     1681 ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt
     1741 ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc
     1801 catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt
     1861 atccagccct cactccttct ctaggcgccc ccatatggcc atatgagatc ttatatgggg
     1921 cacccccgcc ccttgtaaac ttccctgacc ctgacatgac aagagttact aacagcccct
     1981 ctctccaagc tcacttacag gctctctact tagtccagca cgaagtctgg agacctctgg
     2041 cggcagccta ccaagaacaa ctggaccgac cggtggtacc tcacccttac cgagtcggcg
     2101 acacagtgtg ggtccgccga caccagacta agaacctaga acctcgctgg aaaggacctt
     2161 acacagtcct gctgaccacc cccaccgccc tcaaagtaga cggcatcgca gcttggatac
     2221 acgccgccca cgtgaaggct gccgaccccg ggggtggacc atcctctagc cctcgagttt
     2281 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     2341 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     2401 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     2461 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     2521 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gctcggtacc
     2581 cgggtcgagg taggcgtgta cggtgggagg cctatataag cagagctcgt ttagtgaacc
     2641 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc
     2701 gatccgacct gccatgggca gcagccatca tcatcatcat cacagcagcg gcctggtgcc
     2761 gcgcggcagc catatggccc aagttgcaat gtccaccctc cccgttgaag atgaggagtc
     2821 ctcggagagc aggatggtgg tgacattcct catgtcagct ctcgagtcca tgtgtaaaga
     2881 actggccaag tccaaagccg aagtggcctg cattgcagtg tatgaaacag acgtgtttgt
     2941 cgtcggaact gaaagaggac gtgcttttgt caataccaga aaggattttc aaaaagattt
     3001 tgtaaaatat tgtgttgaag aagaagaaaa agctgcagag atgcataaaa tgaaatctac
     3061 aacccaggca aatcggatga gtgtagatgc tgtagaaatt gaaacactca gaaaaacagt
     3121 tgaggactat ttctgctttt gctatgggaa agctttaggc aaatccacag tggtacctgt
     3181 accatatgag aagatgctgc gagaccagtc ggctgtggta gtgcaggggc ttccggaagg
     3241 tgttgccttt aaacaccccg agaactatga tcttgcaacc ctgaaatgga ttttggagaa
     3301 caaagcagga atttcattca tcattaagag acctttttta gagccaaaga agcatgtagg
     3361 tggtcgtgtg atggtaacag atgctgacag gtcaatacta tctccaggtg gaagttgtgg
     3421 ccccatcaaa gtgaaaactg aacccacaga agattctggc atttccctgg aaatggcagc
     3481 tgtgacagta aaggaagaat cagaagatcc tgattattat caatataaca ttcaaggaag
     3541 ccaccattct tcagagggca atgaaggcac agaaatggaa gtaccagcag aagatgatga
     3601 ttattctcca ccgtctaaga gaccaaaggc caatgagcta ccgcagccac cagtcccgga
     3661 acccgccaat gctgggaagc ggaaagtgag ggagttcaac ttcgagaaat ggaatgctcg
     3721 catcactgat ctacgtaaac aagttgaaga attgtttgaa aggaaatatg ctcaagccat
     3781 aaaagccaaa ggtccggtga cgatcccgta ccctcttttc cagtctcatg ttgaagatct
     3841 ttatgtagaa ggacttcctg aaggaattcc ttttaaaagg ccatctactt acggaattcc
     3901 tcgcctggag aggatattac ttgcaaagga aaggattcgt tttgtgatta agaaacatga
     3961 gcttctgaat tcaacacgtg aagatttaca gcttgataag ccagcttcag gagtaaagga
     4021 agaatggtat gccagaatca ctaaattaag aaagatggtg gatcagcttt tctgcaaaaa
     4081 atttgcggaa cgcttgggga gcactgaagc caaggctgta ccgtaccaaa aatttgaggc
     4141 acacccgaat gatctgtacg tggaaggact gccagaaaac attcctttcc gaagtccctc
     4201 atggtatgga atcccaaggc tggaaaaaat cattcaagtg ggcaatcgaa ttaaatttgt
     4261 tattaaaaga ccagaacttc tgactcacag taccactgaa gttactcagc caagaacgaa
     4321 tacaccagtc aaagaagatt ggaatgtcag aattaccaag ctacggaagc aagtggaaga
     4381 gatttttaat ttgaaatttg ctcaagctct tggactcacc gaggcagtaa aagtaccata
     4441 tcctgtgttt gaatcaaacc cggagttctt gtatgtggaa ggcttgccag aggggattcc
     4501 cttccgaagc cctacctggt ttggaattcc acgacttgaa aggatcgtcc gcgggagtaa
     4561 taaaatcaag ttcgttgtta aaaaacctga actagttatt tcctacttgc ctcctgggat
     4621 ggctagtaaa ataaacacta aagctttgca gtcccccaaa agaccacgaa gtcctgggag
     4681 taattcaaag gttcctgaaa ttgaggtcac cgtggaaggc cctaataaca acaatcctca
     4741 aacctcagct gttcgaaccc cgacccagac taacggttct aacgttccct tcaagccacg
     4801 agggagagag ttttcctttg aggcctggaa tgccaaaatc acggacctaa aacagaaagt
     4861 tgaaaatctc ttcaatgaga aatgtgggga agctcttggc cttaaacaag ctgtgaaggt
     4921 gccgttcgcg ttatttgagt ctttcccgga agacttttat gtggaaggct tacctgaggg
     4981 tgtgccattc cgaagaccat cgacttttgg cattccgaga ctggagaaga tactcagaaa
     5041 caaagccaaa attaagttca tcattaaaaa gcccgaaatg tttgagacgg cgattaagga
     5101 gagcacctcc tctaagagcc ctcccagaaa aataaattca tcacccaatg ttaatactac
     5161 tgcatcaggt gttgaagacc ttaacatcat tcaggtgaca attccagatg atgataatga
     5221 aagactctcg aaagttgaaa aagctagaca gctaagagaa caagtgaatg acctctttag
     5281 tcggaaattt ggtgaagcta ttggtatggg ttttcctgtg aaagttccct acaggaaaat
     5341 cacaattaac cctggctgtg tggtggttga tggcatgccc ccgggggtgt ccttcaaagc
     5401 ccccagctac ctggaaatca gctccatgag aaggatctta gactctgccg agtttatcaa
     5461 attcacggtc attagaccat ttccaggact tgtgattaat aaccagctgg ttgatcagag
     5521 tgagtcagaa ggccccgtga tacaagaatc agctgaacca agccagttgg aagttccagc
     5581 cacagaagaa ataaaagaga ctgatggaag ctctcagatc aagcaagaac cagaccccac
     5641 gtggtcgacg gtaccgcggg cccgggatcc accggtcgcc accatggtga gcaagggcga
     5701 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     5761 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     5821 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     5881 ccacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     5941 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     6001 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     6061 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaactt
     6121 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     6181 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     6241 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     6301 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     6361 cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc gcggatcccc
     6421 cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     6481 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     6541 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     6601 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     6661 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     6721 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     6781 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     6841 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     6901 catgctttac atgtgtttag tcgaggttaa aaaacgtcta ggccccccga accacgggga
     6961 cgtggttttc ctttgaaaaa cacgatgata agcttgccac aaccatgtct agattagata
     7021 aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa
     7081 caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa
     7141 aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc
     7201 acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt
     7261 ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta
     7321 cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt
     7381 cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat
     7441 tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta
     7501 tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct
     7561 tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg
     7621 ggtccgcgta cagccgcgcg cgtacgaaaa acaattacgg gtctaccatc gagggcctgc
     7681 tcgatctccc ggacgacgac gcccccgaag aggcggggct ggcggctccg cgcctgtcct
     7741 ttctccccgc gggacacacg cgcagactgt cgacggcccc cccgaccgat gtcagcctgg
     7801 gggacgagct ccacttagac ggcgaggacg tggcgatggc gcatgccgac gcgctagacg
     7861 atttcgatct ggacatgttg ggggacgggg attccccggg tccgggattt accccccacg
     7921 actccgcccc ctacggcgct ctggatatgg ccgacttcga gtttgagcag atgtttaccg
     7981 atgcccttgg aattgacgag tacggtgggt agggggcgcg aagatccgga ttagtccaat
     8041 ttgttaaaga caggatatca gtggtccagg ctctagtttt gactcaacaa tatcaccagc
     8101 tgaagcctat agagtacgag ccatagataa aataaaagat tttatttagt ctccagaaaa
     8161 aggggggaat gaaagacccc acctgtaggt ttggcaagct agctcgaccc tagagaacca
     8221 tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac
     8281 caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag
     8341 cccacaaccc ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt
     8401 gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg
     8461 gtctcctctg agtgattgac tacccgtcag cgggggtctt tcacacatgc agcatgtatc
     8521 aaaattaatt tggttttttt tcttaagtat ttacattaaa tggccatagt acttaaagtt
     8581 acattggctt ccttgaaata aacatggagt attcagaatg tgtcataaat atttctaatt
     8641 ttaagatagt atctccattg gctttctact ttttctttta tttttttttg tcctctgtct
     8701 tccatttgtt gttgttgttg tttgtttgtt tgtttgttgg ttggttggtt aatttttttt
     8761 taaagatcct acactatagt tcaagctaga ctattagcta ctctgtaacc cagggtgacc
     8821 ttgaagtcat gggtagcctg ctgttttagc cttcccacat ctaagattac aggtatgagc
     8881 tatcattttt ggtatattga ttgattgatt gattgatgtg tgtgtgtgtg attgtgtttg
     8941 tgtgtgtgac tgtgaaaatg tgtgtatggg tgtgtgtgaa tgtgtgtatg tatgtgtgtg
     9001 tgtgagtgtg tgtgtgtgtg tgtgcatgtg tgtgtgtgtg actgtgtcta tgtgtatgac
     9061 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgttgt gaaaaaatat
     9121 tctatggtag tgagagccaa cgctccggct caggtgtcag gttggttttt gagacagagt
     9181 ctttcactta gcttggaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     9241 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     9301 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
     9361 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
     9421 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     9481 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     9541 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgatgac
     9601 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     9661 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     9721 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     9781 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     9841 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     9901 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     9961 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
    10021 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
    10081 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
    10141 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
    10201 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
    10261 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
    10321 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
    10381 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
    10441 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
    10501 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
    10561 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
    10621 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
    10681 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    10741 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    10801 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    10861 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    10921 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
    10981 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    11041 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    11101 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    11161 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    11221 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    11281 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    11341 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    11401 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    11461 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    11521 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    11581 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
    11641 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
    11701 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
    11761 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
    11821 atgattacgc c
//
