LOCUS       sequence-101662-        8626 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for RFP657 NHEJ reporter system expression. Allows
            insertion of target sites between the SFFV promoter and RFP657 for 
            detection of fluorescence knockdown. Puromycin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8626)
  AUTHORS   Heckl D, Kowalczyk MS, Yudovich D, Belizaire R, Puram RV, McConkey 
            ME, Thielke A, Aster JC, Regev A, Ebert BL
  TITLE     Generation of mouse models of myeloid malignancy with combinatorial 
            genetic lesions using CRISPR-Cas9 genome editing.
  JOURNAL   Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951.
  PUBMED    24952903
REFERENCE   2  (bases 1 to 8626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951."
COMMENT     Sequence Label: pL-SFFV.Reporter.RFP657.PAC
FEATURES             Location/Qualifiers
     source          1..8626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             244..424
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    468..593
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1086..1319
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1504..1548
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1815..1932
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1966..2372
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             2437..3135
                     /codon_start=1
                     /product="far-red monomeric fluorescent protein with an 
                     emission peak at 657 nm (Morozova et al., 2010)"
                     /label=TagRFP657
                     /note="derived from mKate"
                     /translation="SELITENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTQRIKVV
                     EGGPLPFAFDILATSFMYGSHTFINHTQGIPDFWKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEAHTEMLYPADGGLEGRTALALKL
                     VGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDYRLERIKEADKETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     misc_feature    join(3159..3733,3734..3736,3737..3745)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3326..3343)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3553..3572
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3755..4354
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3755..3774)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4251..4271
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4597..5000
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             5090..5323
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5395..5516
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5432..5451)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5486..5505
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5535..5670
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5702..5721)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5703..5721)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5731..5748)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5731..5747)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5740..5762)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5892..6347
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5979..5998)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6189..6210
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6376..6480
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6481..6549,6550..7341)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6699..6718)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7512..8100
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8001..8020
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8254..8271
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8388..8409
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8424..8429,8430..8447,8448..8454)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8462..8478
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8467..8489
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8486..8502
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8486..8502
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8521..8541
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8523..8541
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 cgagttagag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
       61 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
      121 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
      181 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata
      241 aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      301 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      361 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
      421 agcagtggcg cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca
      481 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      541 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      601 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      661 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      721 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      781 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      841 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      901 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      961 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     1021 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     1081 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc
     1141 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     1201 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1261 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1321 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1381 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1441 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1501 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1561 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1621 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1681 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1741 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1801 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     1861 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1921 attcaaaatt ttatcgatca cgagactagc ctcgagctag ctgcagtaac gccattttgc
     1981 aaggcatgga aaaataccaa accaagaata gagaagttca gatcaagggc gggtacatga
     2041 aaatagctaa cgttgggcca aacaggatat ctgcggtgag cagtttcggc cccggcccgg
     2101 ggccaagaac agatggtcac cgcagtttcg gccccggccc gaggccaaga acagatggtc
     2161 cccagatatg gcccaaccct cagcagtttc ttaagaccca tcagatgttt ccaggctccc
     2221 ccaaggacct gaaatgaccc tgcgccttat ttgaattaac caatcagcct gcttctcgct
     2281 tctgttcgcg cgcttctgct tcccgagctc tataaaagag ctcacaaccc ctcactcggc
     2341 gcgccagtcc tccgacagac tgagtcggcc ggtcgaatca agcttatcga taccgtcgac
     2401 ggatccaccg gtgccaccat gtccggagcc acgcgtagcg agctgatcac cgagaacatg
     2461 cacatgaagc tgtacatgga gggcaccgtg aacaaccacc acttcaagtg cacatccgag
     2521 ggcgaaggca agccctacga gggcacccag acccagagaa tcaaggtggt cgagggcggc
     2581 cctctcccct tcgccttcga catcctggct accagcttca tgtacggcag tcacaccttc
     2641 atcaaccaca cccagggcat ccccgacttc tggaagcagt ccttccctga gggcttcaca
     2701 tgggagagag tcaccacata cgaagacggg ggcgtgctga ccgctaccca ggacaccagc
     2761 ctccaggacg gctgcctcat ctacaacgtc aagatcagag gggtgaactt cccatccaac
     2821 ggccctgtga tgcagaagaa aacactcggc tgggaggccc acaccgagat gctgtacccc
     2881 gctgacggcg gcctggaagg cagaaccgcg ctggccctga agctcgtggg cgggggccac
     2941 ctgatctgca acttcaagac cacatacaga tccaagaaac ccgctaagaa cctcaagatg
     3001 cccggcgtct actatgtgga ctacagactg gaaagaatca aggaggccga caaagagacc
     3061 tacgtcgagc agcacgaggt ggctgtggcc agatactgcg acctccctag caaactgggg
     3121 cacaaactta attgaatgca tgagtaactg aggatccgcc cctctccctc ccccccccct
     3181 aacgttactg gccgaagccg cttgaaataa ggccggtgtg cgtttgtcta tatgttattt
     3241 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     3301 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     3361 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     3421 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     3481 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     3541 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     3601 gtaccccatt gtatgggatc tgatctgggg cctcggtaca catgctttac atgtgtttag
     3661 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     3721 acacgatgat aatatggcca caaccctggc caccatgacc gagtacaagc ccacggtgcg
     3781 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     3841 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     3901 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     3961 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     4021 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     4081 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     4141 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     4201 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     4261 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     4321 cacctggtgc atgacccgca agcccggtgc ctgatgtaca agtaaagcgg ccgcgtcgac
     4381 ggatcccccg ggctgcagga attcgagcat cttaccgcca tttattccca tatttgttct
     4441 gtttttcttg atttgggtat acatttaaat gttaataaaa caaaatggtg gggcaatcat
     4501 ttacattttt agggatatgt aattactagt tcaggtgtat tgccacaaga caaacatgtt
     4561 aagaaacttt cccgttattt acgctctgtt cctgttaatc aacctctgga ttacaaaatt
     4621 tgtgaaagat tgactgatat tcttaactat gttgctcctt ttacgctgtg tggatatgct
     4681 gctttaatgc ctctgtatca tgctattgct tcccgtacgg ctttcgtttt ctcctccttg
     4741 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtccg tcaacgtggc
     4801 gtggtgtgct ctgtgtttgc tgacgcaacc cccactggct ggggcattgc caccacctgt
     4861 caactccttt ctgggacttt cgctttcccc ctcccgatcg ccacggcaga actcatcgcc
     4921 gcctgccttg cccgctgctg gacaggggct aggttgctgg gcactgataa ttccgtggtg
     4981 ttgtcgggga agctgacgtc ctttcgaatt cgatatcagg tacctttaag accaatgact
     5041 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     5101 cgtaactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     5161 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     5221 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     5281 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     5341 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     5401 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     5461 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     5521 gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     5581 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     5641 agaagtagtg aggaggcttt tttggaggcc taggcttttg cgtcgagacg tacccaattc
     5701 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     5761 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     5821 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     5881 cgaatggcgc gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     5941 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     6001 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     6061 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     6121 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     6181 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     6241 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     6301 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttccc aggtggcact
     6361 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     6421 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     6481 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     6541 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     6601 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     6661 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     6721 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     6781 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     6841 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     6901 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     6961 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     7021 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     7081 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     7141 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     7201 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     7261 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     7321 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     7381 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     7441 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     7501 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     7561 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     7621 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     7681 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     7741 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     7801 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     7861 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     7921 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     7981 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     8041 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     8101 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     8161 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     8221 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     8281 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     8341 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     8401 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     8461 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
     8521 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aaataccacc agaaactgtt
     8581 attcaacaac gacaacagaa tattaaacgt tagagattag ttcaaa
//
